1887

Abstract

A facultatively alkaliphilic, lactic-acid-producing and halophilic strain, designated SA9, was isolated from a fermented indigo ( Lour.) liquor sample prepared in a laboratory. The 16S rRNA gene sequence phylogeny suggested that strain SA9 was a member of the genus with the closest relative being KCCM 42318 (99.3 % 16S rRNA gene sequence similarity). Cells of strain SA9 stained Gram-positive and were facultative anaerobic straight rods that were motile by peritrichous flagella. The strain grew between 5 and 48 °C (optimum, 35 °C) and at pH 7–12 (optimum, pH 9). The isoprenoid quinone detected was menaquinone-7 (MK-7) and the DNA G+C content was 40.6±0.9 mol%. The whole-cell fatty acid profile mainly consisted of iso-C, anteiso-C, C and anteiso-C. DNA–DNA hybridization with DSM 18246 revealed a DNA–DNA relatedness value of 23±2 %. On the basis of the differences in phenotypic and chemotaxonomic characteristics, and the results of phylogenetic analyses based on 16S rRNA gene sequences and DNA–DNA relatedness data from recognized species of the genus , strain SA9 merits classification as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SA9 ( = JCM 17252 = NCIMB 14684). An emended description of the genus is also provided.

Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award 23570128)
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2013-09-01
2024-03-29
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