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Abstract

A strictly aerobic, Gram-staining-negative, rod-shaped, non-motile, yellow-pigmented bacterial strain, designated BX12, was isolated from soil collected from the peak area of Wudang Mountain in the city of Shiyan, Hubei province, China. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain BX12 was most closely related to WB 4.1-42 (95.9 % 16S rRNA gene sequence similarity), followed by F44-8 (95.6 %) and WB 3.3-2 (94.1 %). The major fatty acids (≥5 %) of strain BX12 were summed feature 3 (comprising Cω7 and/or Cω6), iso-C, C, iso-C 3-OH, and C 3-OH. The major polar lipid was phosphatidylethanolamine, and the major respiratory quinone was menaquinone-6. The genomic DNA G+C content was 43.9 mol%. On the basis of a high number of phenotypic differentiating properties and phylogenetic uniqueness, strain BX12 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is BX12 ( = CCTCC AB 2012197 = KCTC 32147). Emended descriptions of , and are also proposed.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31170106)
  • 863 project (Award 2012AA101402-3)
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2013-09-01
2024-04-19
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References

  1. Ali Z., Cousin S., Frühling A., Brambilla E., Schumann P., Yang Y., Stackebrandt E. ( 2009 ). Flavobacterium rivuli sp. nov., Flavobacterium subsaxonicum sp. nov., Flavobacterium swingsii sp. nov. and Flavobacterium reichenbachii sp. nov., isolated from a hard water rivulet. . Int J Syst Evol Microbiol 59, 26102617. [View Article] [PubMed]
    [Google Scholar]
  2. Bergey D. H., Harrison F. C., Breed R. S., Hammer B. W., Huntoon F. M. (editors) ( 1923 ). Genus II. Flavobacterium gen. nov. . In Bergey’s Manual of Determinative Bacteriology, pp. 97117. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  3. Bernardet J. F. ( 2011 ). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey's Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 106111. Edited by Whitman W. . Baltimore:: The Williams & Wilkins Co;.
    [Google Scholar]
  4. Bernardet J. F., Bowman J. P. ( 2006 ). The genus Flavobacterium . . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 7, pp. 481531. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer;.
    [Google Scholar]
  5. Bernardet J. F., Bowman J. P. ( 2011 ). Genus I. Flavobacterium Bergey et al. 1923. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 112154. Edited by Whitman W. . Baltimore:: The Williams & Wilkins Co;.
    [Google Scholar]
  6. Bernardet J. F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. ( 1996 ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). . Int J Syst Bacteriol 46, 128148. [View Article]
    [Google Scholar]
  7. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [View Article] [PubMed]
    [Google Scholar]
  8. Breznak J. A., Costilow R. N. ( 1994 ). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  9. Collins M. D., Jones D. ( 1980 ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. . J Appl Microbiol 48, 459470. [View Article]
    [Google Scholar]
  10. Dong X.-Z., Cai M.-Y. ( 2001 ). Determinative Manual for Routine Bacteriology. . Beijing:: Scientific Press;.
    [Google Scholar]
  11. Dong K., Chen F., Du Y., Wang G.-J. ( 2013 ). Flavobacterium enshiense sp. nov., isolated from soil and emended descriptions of the genus Flavobacterium and Flavobacterium cauense, Flavobacterium saliperosum and Flavobacterium suncheonense . . Int J Syst Evol Microbiol 63, 886892. [View Article] [PubMed]
    [Google Scholar]
  12. Dussault H. P. ( 1955 ). An improved technique for staining red halophilic bacteria. . J Bacteriol 70, 484485.[PubMed]
    [Google Scholar]
  13. Fan H., Su C., Wang Y., Yao J., Zhao K., Wang Y., Wang G. ( 2008 ). Sedimentary arsenite-oxidizing and arsenate-reducing bacteria associated with high arsenic groundwater from Shanyin, Northwestern China. . J Appl Microbiol 105, 529539. [View Article] [PubMed]
    [Google Scholar]
  14. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  15. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  16. Fu Y., Tang X., Lai Q., Zhang C., Zhong H., Li W., Liu Y., Chen L., Sun F., Shao Z. ( 2011 ). Flavobacterium beibuense sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 61, 205209. [View Article] [PubMed]
    [Google Scholar]
  17. Guindon S., Lethiec F., Duroux P., Gascuel O. ( 2005 ). phyml Online–a web server for fast maximum likelihood-based phylogenetic inference. . Nucleic Acids Res 33 (Web Server issue), W557W559. [View Article] [PubMed]
    [Google Scholar]
  18. Hugh R., Leifson E. ( 1953 ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. . J Bacteriol 66, 2426.[PubMed]
    [Google Scholar]
  19. Jorgensen J. H., Ferraro M. J. ( 2009 ). Antimicrobial susceptibility testing: a review of general principles and contemporary practices. . Clin Infect Dis 49, 17491755. [View Article] [PubMed]
    [Google Scholar]
  20. Kaur I., Kaur C., Khan F., Mayilraj S. ( 2012 ). Flavobacterium rakeshii sp. nov., isolated from marine sediment, and emended description of Flavobacterium beibuense Fu et al. 2011. . Int J Syst Evol Microbiol 62, 28972902. [View Article] [PubMed]
    [Google Scholar]
  21. Kim B.-Y., Weon H.-Y., Cousin S., Yoo S.-H., Kwon S.-W., Go S.-J., Stackebrandt E. ( 2006 ). Flavobacterium daejeonense sp. nov. and Flavobacterium suncheonense sp. nov., isolated from greenhouse soils in Korea. . Int J Syst Evol Microbiol 56, 16451649. [View Article] [PubMed]
    [Google Scholar]
  22. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.-S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  23. Kroppenstedt R. M. ( 1985 ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics (SAB Technical Series no. 20), pp. 173199. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  24. Liu Y., Jin J.-H., Zhou Y.-G., Liu H.-C., Liu Z.-P. ( 2010 ). Flavobacterium caeni sp. nov., isolated from a sequencing batch reactor for the treatment of malachite green effluents. . Int J Syst Evol Microbiol 60, 417421. [View Article] [PubMed]
    [Google Scholar]
  25. Mesbah M., Premachandran U., Whitman W.-B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  26. Minnikin D. E., Collins M. D., Goodfellow M. ( 1979 ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47, 8795. [View Article]
    [Google Scholar]
  27. Nedashkovskaya O. I., Kim S. B., Suzuki M., Shevchenko L. S., Lee M. S., Lee K. H., Park M. S., Frolova G. M., Oh H. W. & other authors ( 2005 ). Pontibacter actiniarum gen. nov., sp. nov., a novel member of the phylum ‘Bacteroidetes’, and proposal of Reichenbachiella gen. nov. as a replacement for the illegitimate prokaryotic generic name Reichenbachia Nedashkovskaya et al. 2003. . Int J Syst Evol Microbiol 55, 25832588. [View Article] [PubMed]
    [Google Scholar]
  28. Reichenbach H. ( 1992 ). The order Cytophagales . . In The Prokaryotes, , 2nd edn., vol. 4, pp. 36313675. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  29. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  30. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
    [Google Scholar]
  31. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  32. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  33. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  34. Weon H.-Y., Song M.-H., Son J.-A., Kim B.-Y., Kwon S. W., Go S.-J., Stackebrandt E. ( 2007 ). Flavobacterium terrae sp. nov. and Flavobacterium cucumis sp. nov., isolated from greenhouse soil. . Int J Syst Evol Microbiol 57, 15941598. [View Article] [PubMed]
    [Google Scholar]
  35. Xie C. H., Yokota A. ( 2003 ). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49, 345349. [View Article] [PubMed]
    [Google Scholar]
  36. Xu M., Xin Y., Tian J., Dong K., Yu Y., Zhang J., Liu H., Zhou Y. ( 2011 ). Flavobacterium sinopsychrotolerans sp. nov., isolated from a glacier. . Int J Syst Evol Microbiol 61, 2024. [View Article] [PubMed]
    [Google Scholar]
  37. Yang J. E., Kim S. Y., Im W. T., Yi T. H. ( 2011 ). Flavobacterium ginsenosidimutans sp. nov., a bacterium with ginsenoside converting activity isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 61, 14081412. [View Article] [PubMed]
    [Google Scholar]
  38. Yoon J. H., Kang S. J., Oh T. K. ( 2006 ). Flavobacterium soli sp. nov., isolated from soil. . Int J Syst Evol Microbiol 56, 9971000. [View Article] [PubMed]
    [Google Scholar]
  39. Yoon J. H., Kang S. J., Lee J. S., Oh T. K. ( 2007 ). Flavobacterium terrigena sp. nov., isolated from soil. . Int J Syst Evol Microbiol 57, 947950. [View Article] [PubMed]
    [Google Scholar]
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