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Abstract

A Gram-stain-negative, rod-shaped, orange-coloured, catalase- and oxidase-positive, non-motile bacterium, designated strain 92V, was isolated from the marine sponge , collected from Lough Hyne, County Cork, Ireland. 16S rRNA gene sequence analysis revealed that strain 92V clustered with members of the family , the closest member being NCIMB 1399, with a gene sequence similarity of 97.5 %. Strain 92V required seawater for growth with optimal growth occurring at 25 °C, at pH 6–7 and with 3 % (w/v) NaCl. MK-6 was the sole respiratory quinone present and the major fatty acids were iso-C 3-OH, iso-C, iso-Cω9 and iso-C 3-OH. The DNA G+C content was 36.1 mol%. Combined phenotypic differences and phylogenetic analysis indicate that strain 92V represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 92V ( = NCIMB 14723 = DSM 25232).

Funding
This study was supported by the:
  • Beaufort Marine Research Award
  • The Marine Institute
  • Science Foundation of Ireland (Award BMT2350, GEN1319, GEN1295 and 07/IN.B948)
  • Environmental Protection Agency (Award EPA 2008-PhD-S-2)
  • European Commission (Award O36314)
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2014-02-01
2024-03-29
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