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Volume 63, Issue Pt_9

sp. nov., a halophilic bacterium from a hypersaline lake Free

Abstract

A Gram-staining-positive, endospore-forming, rod-shaped, strictly aerobic, slightly halophilic bacterium, designated strain A24, was isolated from the hypersaline lake Aran-Bidgol in Iran. Cells of strain A24 were motile rods and produced oval endospores at a terminal position in swollen sporangia. Strain A24 was catalase and oxidase positive. Growth occurred with between 0.5 and 7.5 % (w/v) NaCl and the isolate grew optimally at 3 % (v/w) NaCl. The optimum temperature and pH for growth were 35 °C and pH 8.0, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A24 belonged to the genus within the phylum and showed the closest phylogenetic similarity with the species BA288 (97.2 %), D-1,5a (96.0 %) and SW-211 (95.6 %). The G+C content of the genomic DNA of this strain was 35.9 mol%. The polar lipid pattern of strain A24 consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and two unknown phospholipids. The major cellular fatty acids of strain A24 were anteiso-C and iso-C. The respiratory quinones were MK-7 (94 %) and MK-6 (4 %). The peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. All these features confirm the placement of isolate A24 within the genus . DNA–DNA hybridization experiments revealed a relatedness of 8 % between strain A24 and IBRC-M 10596, supporting its placement as a novel species. Phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data suggest that this strain represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain A24 ( = IBRC-M 10078  = KCTC 13816).

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Funding
This study was supported by the:
  • Iranian Biological Resource Centre (IBRC) (Award MI-1388-04)
  • International Foundation for Science (IFS) (Award A/4527-1)
  • Spanish Ministerio de Economía y Competitividad (Award CGL2010-19303)
  • National Science Foundation (Award  DEB-0919290)
  • Junta de Andalucía (Award P10-CVI-6226)
© 2013 IUMS
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2013-09-01
2024-04-19
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Bacillussalsus sp. nov., a halophilic bacterium from a hypersaline lake
Int J Syst Evol Microbiol 63, 3324 (2013); https://doi.org/10.1099/ijs.0.050120-0
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