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Volume 64, Issue Pt_2

gen. nov., sp. nov., a non-photosynthetic bacterium isolated from soil, and emended descriptions of the genera and Free

Abstract

A Gram-reaction-negative, facultatively anaerobic, non-motile, rod-shaped, non-photosynthetic bacterial strain, DW2-9, was isolated from soil. The highest 16S rRNA gene sequence similarities were found to ATCC 11166 (97.1 %), JA737 (96.4 %), JA276 (96.2 %), ATCC 35703 (96.0 %), CCUG 47968 (96.0 %), CCUG 52307 (95.9 %) and JA296 (95.7 %). The genomic DNA G+C content was 67.2 mol% and the major respiratory quinone was ubiquinone 10 (Q-10). The major cellular fatty acids (>5 %) were Cω7, C, C cyclo ω8 and summed feature 3 (one or more of iso-C 2-OH, Cω6 and Cω7). However, unlike species of the genus , strain DW2-9 neither formed internal photosynthetic membranes nor produced photosynthetic pigments. DNA–DNA hybridization between strain DW2-9 and JCM 21090 showed a relatedness of 33 %. Strain DW2-9 contained phosphatidylethanolamine, phosphatidylglycerol and an unknown aminophospholipid as major polar lipids, which differed from those of species of the genera and . In addition to the differences in phylogenetic position and polar lipid types, strain DW2-9 could be distinguished from species of the genus by the cultivation conditions. On the basis of our polyphasic taxonomic analysis, strain DW2-9 is considered to represent a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is DW2-9 ( = CCTCC AB 2011145 = KCTC 15169). Emended descriptions of the genera and are also proposed.

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Funding
This study was supported by the:
  • Chinese 863 project (Award 2012AA101402-3)
  • Hubei Tobacco Industrial Corporation Ltd, Enshi Branch, PR China
© 2014 IUMS
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2014-02-01
2024-04-25
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Paenirhodobacter enshiensis gen. nov., sp. nov., a non-photosynthetic bacterium isolated from soil, and emended descriptions of the genera Rhodobacter and Haematobacter
Int J Syst Evol Microbiol 64, 551 (2014); https://doi.org/10.1099/ijs.0.050351-0
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