RT Journal Article SR Electronic(1) A1 Toro, Marcia A1 Ramírez-Bahena, Martha-Helena A1 Cuesta, Maria José A1 Velázquez, Encarna A1 Peix, AlvaroYR 2013 T1 Pseudomonas guariconensis sp. nov., isolated from rhizospheric soil JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_12 SP 4413 OP 4420 DO https://doi.org/10.1099/ijs.0.051193-0 PB Microbiology Society, SN 1466-5034, AB We isolated a bacterial strain designated PCAVU11T in the course of a study of phosphate-solubilizing bacteria occurring in rhizospheric soil of Vigna unguiculata (L.) Walp. in Guárico state, Venezuela. The 16S rRNA gene sequence had 99.2 % sequence similarity with respect to the most closely related species, Pseudomonas taiwanensis , and 99.1 % with respect to Pseudomonas entomophila , Pseudomonas plecoglossicida and Pseudomonas monteilii , on the basis of which PCAVU11T was classified as representing a member of the genus Pseudomonas . Analysis of the housekeeping genes rpoB, rpoD and gyrB confirmed the phylogenetic affiliation and showed sequence similarities lower than 95 % in all cases with respect to the above-mentioned closest relatives. Strain PCAVU11T showed two polar flagella. The respiratory quinone was Q9. The major fatty acids were 16 : 0 (25.7 %), 18 : 1ω7c (20.4 %), 17 : 0 cyclo (11.5 %) and 16 : 1ω7c/15 : 0 iso 2-OH in summed feature 3 (10.8 %). The strain was oxidase-, catalase- and urease-positive, the arginine dihydrolase system was present but nitrate reduction, β-galactosidase production and aesculin hydrolysis were negative. Strain PCAVU11T grew at 44 °C and at pH 10. The DNA G+C content was 61.5 mol%. DNA–DNA hybridization results showed values lower than 56 % relatedness with respect to the type strains of the four most closely related species. Therefore, the results of genotypic, phenotypic and chemotaxonomic analyses support the classification of strain PCAVU11T as representing a novel species of the genus Pseudomonas , which we propose to name Pseudomonas guariconensis sp. nov. The type strain is PCAVU11T ( = LMG 27394T = CECT 8262T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.051193-0