%0 Journal Article %A Killer, J. %A Ročková, Š. %A Vlková, E. %A Rada, V. %A Havlík, J. %A Kopečný, J. %A Bunešová, V. %A Benada, O. %A Kofroňová, O. %A Pechar, R. %A Profousová, I. %T Alloscardovia macacae sp. nov., isolated from the milk of a macaque (Macaca mulatta), emended description of the genus Alloscardovia and proposal of Alloscardovia criceti comb. nov. %D 2013 %J International Journal of Systematic and Evolutionary Microbiology, %V 63 %N Pt_12 %P 4439-4446 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.051326-0 %I Microbiology Society, %X A novel bacterial strain, designated M8T, was isolated from milk of a female macaque bred in captivity. The strain was Gram-stain-positive, anaerobic, irregular coccoid–rod-shaped without catalase activity. Analysis of 16S rRNA gene sequence similarity revealed that the isolate was most closely related to Alloscardovia omnicolens CCUG 31649T (96.4 %) and Metascardovia criceti OMB105T (96.6 %). Sequences of hsp60, fusA, and xfp genes also confirmed that the strain was most closely related to the type strains of A. omnicolens and M. criceti . The isolate produced fructose-6-phosphate phosphoketolase which is in agreement with classification within the family Bifidobacteriaceae . The major fatty acids were C18 : 1ω9c (35.8 %), C16 : 1 (6.2 %) and C14 : 0 (5.7 %). Polar lipid analysis revealed five different glycolipids, two unidentified phospholipids and diphosphatidylglycerol. The peptidoglycan was of the type A4α l-Lys–d-Asp with the presence of d(l)-alanine, d-glutamine, d-asparagine and l-lysine. The DNA G+C content of strain M8T was 50.1 mol%. On the basis of genetic, phylogenetic and phenotypic data, strain M8T represents a novel species of the genus Alloscardovia for which the name Alloscardovia macacae sp. nov. is proposed. The type strain is M8T ( = DSM 24762T = CCM 7944T). In addition, our results also revealed that Alloscardovia omnicolens DSM 21503T and Metascardovia criceti DSM 17774T do not belong to different genera within the family Bifidobacteriaceae . We therefore propose to reclassify Metascardovia criceti as Alloscardovia criceti comb. nov. An emended description of the genus Alloscardovia is also provided. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.051326-0