%0 Journal Article %A Montero-Calasanz, Maria del Carmen %A Göker, Markus %A Rohde, Manfred %A Spröer, Cathrin %A Schumann, Peter %A Busse, Hans-Jürgen %A Schmid, Michael %A Tindall, Brian J. %A Klenk, Hans-Peter %A Camacho, Maria %T Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium %D 2013 %J International Journal of Systematic and Evolutionary Microbiology, %V 63 %N Pt_12 %P 4386-4395 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.052456-0 %I Microbiology Society, %X A novel non-motile, Gram-staining-negative, yellow-pigmented bacterium, designated AG13T, isolated from a rain water pond at a plant nursery in Spain and characterized as a plant-growth-promoting bacterium, was investigated to determine its taxonomic status. The isolate grew best over a temperature range of 15–40 °C, at pH 5.0–8.0 and with 0–4 % (w/v) NaCl. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Chryseobacterium . The DNA G+C content of the novel strain was 37.2 mol%. The strain had a polyamine pattern with sym-homospermidine as the major compound and produced flexirubin-type pigments. MK-6 was the dominant menaquinone and the major cellular fatty acids were iso-C15 : 0, C17 : 1ω9c and iso-C17 : 0 3-OH. The main polar lipids were phosphatidylethanolamine, aminolipids and several unidentified lipids. The 16S rRNA gene showed 92.0–97.2 % sequence similarity with those of the members of the genus Chryseobacterium . Based on chemotaxonomic and phenotypic traits, and DNA–DNA hybridizations with the type strains of the most closely related species, the isolate is proposed to represent a novel species, Chryseobacterium hispalense, type strain AG13T ( = DSM 25574T = CCUG 63019T). Emended descriptions of the species Chryseobacterium defluvii , Chryseobacterium indologenes , Chryseobacterium wanjuense and Chryseobacterium gregarium are also provided. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.052456-0