1887

Abstract

A coccal strain isolated from fresh broccoli was initially identified as ; however, molecular identification and phenotypic traits did not support this identification. DNA–DNA hybridization with the type strain of (76.4 % relatedness), DNA G+C content (35.7 mol%), phylogenetic analysis based on 16S rRNA, and gene sequences, rep-PCR fingerprinting and profiles of cellular fatty acids, whole-cell proteins and enzyme activities, together with carbohydrate metabolism characteristics, indicated that this strain is distinct and represents a novel subspecies, for which the name subsp. subsp. nov. is proposed. The type strain is 812 ( = NBRC 109476 = BCRC 80575). Furthermore, we present an emended description of and proposal of subsp. subsp. nov. (type strain ATCC 43076 = CCUG 27643 = CCUG 33311 = CIP 103246 = DSM 20726 = JCM 8734 = LMG 11427 = NBRC 100493 = NCIMB 702594).

Funding
This study was supported by the:
  • National Science Council Taiwan (Award NSC 101-2313-B-130-001)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.052761-0
2013-12-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/12/4691.html?itemId=/content/journal/ijsem/10.1099/ijs.0.052761-0&mimeType=html&fmt=ahah

References

  1. Ampe F., Sirvent A., Zakhia N. ( 2001 ). Dynamics of the microbial community responsible for traditional sour cassava starch fermentation studied by denaturing gradient gel electrophoresis and quantitative rRNA hybridization. . Int J Food Microbiol 65, 4554. [View Article] [PubMed]
    [Google Scholar]
  2. ben Omar N., Ampe F. ( 2000 ). Microbial community dynamics during production of the Mexican fermented maize dough pozol. . Appl Environ Microbiol 66, 36643673. [View Article] [PubMed]
    [Google Scholar]
  3. Chen Y. S., Yanagida F., Hsu J. S. ( 2006 ). Isolation and characterization of lactic acid bacteria from suan-tsai (fermented mustard), a traditional fermented food in Taiwan. . J Appl Microbiol 101, 125130. [View Article] [PubMed]
    [Google Scholar]
  4. Chen Y. S., Miyashita M., Suzuki K., Sato H., Hsu J. S., Yanagida F. ( 2010 ). Lactobacillus pobuzihii sp. nov., isolated from pobuzihi (fermented cummingcordia). . Int J Syst Evol Microbiol 60, 19141917. [View Article] [PubMed]
    [Google Scholar]
  5. Chen Y. S., Liou M. S., Ji S. H., Yu C. R., Pan S. F., Yanagida F. ( 2013 ). Isolation and characterization of lactic acid bacteria from Yan-tsai-shin (fermented broccoli stems), a traditional fermented food in Taiwan. . J Appl Microbiol 115, 125132. [View Article] [PubMed]
    [Google Scholar]
  6. Dellaglio F., Felis G. E., Castioni A., Torriani S., Germond J. E. ( 2005 ). Lactobacillus delbrueckii subsp. indicus subsp. nov., isolated from Indian dairy products. . Int J Syst Evol Microbiol 55, 401404. [View Article] [PubMed]
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  8. Farrow J. A. E., Kruze J., Phillips B. A., Bramley A. J., Collins M. D. ( 1984 ). Taxonomic studies on Streptococcus bovis and Streptococcus equinus: description of Streptococcus alactolyticus sp. nov. and Streptococcus saccharolyticus sp. nov.. Syst Appl Microbiol 5, 467482. [View Article]
    [Google Scholar]
  9. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  10. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  11. Fitch W. M. ( 1977 ). On the problem of discovering the most parsimonious tree. . Am Nat 111, 223257. [View Article]
    [Google Scholar]
  12. Garrity G. M., Labeda D. P., Oren A. ( 2011 ). Judicial Commission of the International Committee on Systematics of Prokaryotes. XIIth International (IUMS) Congress of Bacteriology and Applied Microbiology. Minutes of the meetings, 3, 4 and 6 August 2008, Istanbul, Turkey. . Int J Syst Evol Microbiol 61, 27752780. [View Article]
    [Google Scholar]
  13. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K. ( 1998 ). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. . Can J Microbiol 44, 11481153. [View Article]
    [Google Scholar]
  14. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  15. Kozaki M., Uchimura T., Okada S. ( 1992 ). In Experimental Manual of Lactic Acid Bacteria, pp. 2972. Tokyo:: Asakurashoten;.
    [Google Scholar]
  16. Layton B. A., Walters S. P., Lam L. H., Boehm A. B. ( 2010 ). Enterococcus species distribution among human and animal hosts using multiplex PCR. . J Appl Microbiol 109, 539547.[PubMed]
    [Google Scholar]
  17. Morandi S., Cremonesi P., Povolo M., Brasca M. ( 2012 ). Enterococcus lactis sp. nov., from Italian raw milk cheeses. . Int J Syst Evol Microbiol 62, 19921996. [View Article] [PubMed]
    [Google Scholar]
  18. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J. ( 2005 ). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology 151, 21412150. [View Article] [PubMed]
    [Google Scholar]
  19. Poli A., Guven K., Romano I., Pirinccioglu H., Guven R. G., Euzeby J. P., Matpan F., Acer O., Orlando P., Nicolaus B. ( 2012 ). Geobacillus subterraneus subsp. aromaticivorans subsp. nov., a novel thermophilic and alkaliphilic bacterium isolated from a hot spring in Sırnak, Turkey. . J Gen Appl Microbiol 58, 437446. [View Article] [PubMed]
    [Google Scholar]
  20. Rahkila R., Johansson P., Säde E., Björkroth J. ( 2011 ). Identification of enterococci from broiler products and a broiler processing plant and description of Enterococcus viikkiensis sp. nov.. Appl Environ Microbiol 77, 11961203. [View Article] [PubMed]
    [Google Scholar]
  21. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  22. Sistek V., Maheux A. F., Boissinot M., Bernard K. A., Cantin P., Cleenwerck I., De Vos P., Bergeron M. G. ( 2012 ). Enterococcus ureasiticus sp. nov. and Enterococcus quebecensis sp. nov., isolated from water. . Int J Syst Evol Microbiol 62, 13141320. [View Article] [PubMed]
    [Google Scholar]
  23. Švec P., Vancanneyt M., Devriese L. A., Naser S. M., Snauwaert C., Lefebvre K., Hoste B., Swings J. ( 2005a ). Enterococcus aquimarinus sp. nov., isolated from sea water. . Int J Syst Evol Microbiol 55, 21832187. [View Article] [PubMed]
    [Google Scholar]
  24. Švec P., Vancanneyt M., Seman M., Snauwaert C., Lefebvre K., Sedláček I., Swings J. ( 2005b ). Evaluation of (GTG)5-PCR for identification of Enterococcus spp.. FEMS Microbiol Lett 247, 5963. [View Article] [PubMed]
    [Google Scholar]
  25. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  28. Tyrrell G. J., Turnbull L., Teixeira L. M., Lefebvre J., Carvalho M. G., Facklam R. R., Lovgren M. ( 2002 ). Enterococcus gilvus sp. nov. and Enterococcus pallens sp. nov. isolated from human clinical specimens. . J Clin Microbiol 40, 11401145. [View Article] [PubMed]
    [Google Scholar]
  29. Versalovic J., Schneider M., De Bruijn F. J., Lupski J. R. ( 1994 ). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. . Methods Mol Cell Biol 5, 2540.
    [Google Scholar]
  30. Wang L. T., Lee F. L., Tai C. J., Yokota A., Kuo H. P. ( 2007 ). Reclassification of Bacillus axarquiensis Ruiz-Garcia et al. 2005 and Bacillus malacitensis Ruiz-Garcia et al. 2005 as later heterotypic synonyms of Bacillus mojavensis Roberts et al. 1994. . Int J Syst Evol Microbiol 57, 16631667. [View Article] [PubMed]
    [Google Scholar]
  31. Watts J. L. ( 1989 ). Evaluation of the Rapid Strep system for identification of gram-positive, catalase-negative cocci isolated from bovine intramammary infections. . J Dairy Sci 72, 27282732. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.052761-0
Loading
/content/journal/ijsem/10.1099/ijs.0.052761-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error