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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_12

gen. nov., sp. nov., a cellulolytic and filamentous thermophile that represents a novel lineage ( ord. nov., fam. nov.) within the class Free

Abstract

Several closely related, thermophilic and cellulolytic bacterial strains, designated JKG1, JKG2, JKG3, JKG4 and JKG5, were isolated from a cellulolytic enrichment (corn stover) incubated in the water column of Great Boiling Spring, NV. Strain JKG1 had cells of diameter 0.7–0.9 µm and length ~2.0 µm that formed non-branched, multicellular filaments reaching >300 µm. Spores were not formed and dense liquid cultures were red. The temperature range for growth was 45–65 °C, with an optimum of 55 °C. The pH range for growth was pH 5.6–9.0, with an optimum of pH 7.5. JKG1 grew as an aerobic heterotroph, utilizing glucose, sucrose, xylose, arabinose, cellobiose, CM-cellulose, filter paper, microcrystalline cellulose, xylan, starch, Casamino acids, tryptone, peptone, yeast extract, acetate, citrate, lactate, pyruvate and glycerol as sole carbon sources, and was not observed to photosynthesize. The cells stained Gram-negative. Phylogenetic analysis using 16S rRNA gene sequences placed the new isolates in the class , but distant from other cultivated members, with the highest sequence identity of 82.5 % to . The major quinone was menaquinone-9; no ubiquinones were detected. The major cellular fatty acids (>5 %) were C, anteiso-C, iso-C, iso-C, C, iso-C and C. The peptidoglycan amino acids were alanine, ornithine, glutamic acid, serine and asparagine. Whole-cell sugars included mannose, rhamnose, glucose, galactose, ribose, arabinose and xylose. Morphological, phylogenetic and chemotaxonomic results suggest that JKG1 is representative of a new lineage within the class , which we propose to designate gen. nov., sp. nov., fam. nov., ord. nov. The type strain of gen. nov., sp. nov. is JKG1 ( = DSM 26889 = JCM 19132).

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Funding
This study was supported by the:
  • National Science Foundation (Award OISE-0968421, MCB-0546865, REU DBI-1005223 and EPSCoR RII EPS-0814372)
  • US Department of Energy (Award Nevada Renewable Energy Consortium, EMSL Rapid 47730, JGI CSP-182, Urban 21 and DE-EE-0000716)
  • National Basic Research Program of China (Award 2010CB833801)
  • National Natural Science Foundation of China (Award 31070007)
  • Key Project of International Cooperation of China Ministry of Science & Technology (Award 2013DFA31980)
  • UNLV Foundation
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2013-12-01
2024-04-16
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Kallotenue papyrolyticum gen. nov., sp. nov., a cellulolytic and filamentous thermophile that represents a novel lineage (Kallotenuales ord. nov., Kallotenuaceae fam. nov.) within the class Chloroflexia
Int J Syst Evol Microbiol 63, 4675 (2013); https://doi.org/10.1099/ijs.0.053348-0
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