1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_5

sp. nov., isolated from soil Free

Abstract

Two bacterial strains (JC167 and JC168) were isolated from a soil sample collected from Mandpam, Tamilnadu, India. Colonies of both strains were orange and cells Gram-stain-positive. Cells were small rods, and formed terminal endospores of ellipsoidal to oval shape. Both strains were positive for catalase, oxidase and hydrolysis of starch/gelatin, and negative for chitin hydrolysis, HS production, indole production and nitrate reduction activity. Major fatty acids of both strains (>5 %) were anteiso-C, iso-C, iso-C, anteiso-C, iso-C and C with minor (<5 but >1 %) amounts of iso-C, anteiso-C B/iso-C I and Cω11. Diphosphatydilglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids of both strains. Cell wall amino acids were -alanine, -alanine, -glutamic acid and -diaminopimelic acid. β-Carotene and five unidentified carotenoids were present in both strains. Mean genomic DNA G+C content was 53.4±1 mol% and the two strains were closely related (mean DNA–DNA hybridization >90 %). 16S rRNA gene sequence comparisons of both strains indicated that they represent species of the genus within the family of the phylum . Both strains had a sequence similarity of 97.6 % with 6AG and <96.8 % with other members of the genus . Sequence similarity between strain JC167 and 168 was 100 %. Strain JC167 showed 25.8±1 % reassociation (based on DNA–DNA hybridization) with DSM 15402 ( = 6AG). Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain JC167 as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC167 ( = KCTC 33100 = LMG 27257).

  • Published Online:
Funding
This study was supported by the:
  • CSIR, Government of India
© 2014 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.053504-0
2014-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/5/1580.html?itemId=/content/journal/ijsem/10.1099/ijs.0.053504-0&mimeType=html&fmt=ahah

References

  1. Amoozegar M. A., Didari M., Bagheri M., Fazeli S. A. S. F., Schumann P., Spröer C., Sánchez-Porro C., Ventosa A. ( 2013 ). Bacillus salsus sp. nov., a halophilic bacterium from a hypersaline lake. . Int J Syst Evol Microbiol 63, 3324–3329. [View Article] [PubMed]
    [Google Scholar]
  2. Arahal D. R., Márquez M. C., Volcani B. E., Schleifer K. H., Ventosa A. ( 1999 ). Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea. . Int J Syst Bacteriol 49, 521–530. [View Article] [PubMed]
    [Google Scholar]
  3. Borchert M. S., Nielsen P., Graeber I., Kaesler I., Szewzyk U., Pape T., Antranikian G., Schäfer T. ( 2007 ). Bacillus plakortidis sp. nov. and Bacillus murimartini sp. nov., novel alkalitolerant members of rRNA group 6. . Int J Syst Evol Microbiol 57, 2888–2893. [View Article] [PubMed]
    [Google Scholar]
  4. Caccamo D., Gugliandolo C., Stackebrandt E., Maugeri T. L. ( 2000 ). Bacillus vulcani sp. nov., a novel thermophilic species isolated from a shallow marine hydrothermal vent. . Int J Syst Evol Microbiol 50, 2009–2012. [View Article] [PubMed]
    [Google Scholar]
  5. Cappuccino J. G., Sherman N. ( 1998 ). Microbiology - A Laboratory Mannual, , 5th edn.. Menlo Park:: Benjamin/Cummings;.
     [Google Scholar]
  6. Carrasco I. J., Márquez M. C., Xue Y., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A. ( 2007 ). Bacillus chagannorensis sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China. . Int J Syst Evol Microbiol 57, 2084–2088. [View Article] [PubMed]
    [Google Scholar]
  7. Chakravarthy S. K., Ramaprasad E. V. V., Shobha E., Sasikala Ch., Ramana ChV. ( 2012 ). Rhodoplanes piscinae sp. nov. isolated from pond water. . Int J Syst Evol Microbiol 62, 2828–2834. [View Article] [PubMed]
    [Google Scholar]
  8. Hiraishi A., Hoshino Y., Kitamura H. ( 1984 ). Isoprenoid quinone composition in the classification of Rhodospirillaceae . . J Gen Appl Microbiol 30, 197–210. [View Article]
    [Google Scholar]
  9. Hugh R., Leifson E. ( 1953 ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram negative bacteria. . J Bacteriol 66, 24–26.[PubMed]
    [Google Scholar]
  10. Kates M. ( 1986 ). Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids. Amsterdam:: Elsevier;.
     [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  12. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  13. Kosowski K., Schmidt M., Pukall R., Hause G., Kämpfer P., Lechner U. ( 2014 ). Bacillus pervagus sp. nov. and Bacillus andreesenii sp. nov., isolated from a composting reactor. . Int J Syst Evol Microbiol 64, 88–94. [View Article] [PubMed]
    [Google Scholar]
  14. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. ( 1985 ). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. . Proc Natl Acad Sci U S A 82, 6955–6959. [View Article] [PubMed]
    [Google Scholar]
  15. Logan N. A., de Vos P. ( 2009 ). Genus Bacillus . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 21–127. Edited by de Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B. . New York:: Springer;.
     [Google Scholar]
  16. Logan N. A., Berge O., Bishop A. H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. & other authors ( 2009 ). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59, 2114–2121. [View Article] [PubMed]
    [Google Scholar]
  17. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208–218,IN1. [View Article]
    [Google Scholar]
  18. McKerrow J., Vagg S., McKinney T., Seviour E. M., Maszenan A. M., Brooks P., Seviour R. J. ( 2000 ). A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. . Lett Appl Microbiol 30, 178–182. [View Article] [PubMed]
    [Google Scholar]
  19. Meier-Kolthoff J. P., Göker M., Spröer C., Klenk H.-P. ( 2013 ). When should a DDH experiment be mandatory in microbial taxonomy. ? Arch Microbiol 195, 413–418. [View Article] [PubMed]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  21. Pappa A., Sánchez-Porro C., Lazoura P., Kallimanis A., Perisynakis A., Ventosa A., Drainas C., Koukkou A. I. ( 2010 ). Bacillus halochares sp. nov., a halophilic bacterium isolated from a solar saltern. . Int J Syst Evol Microbiol 60, 1432–1436. [View Article] [PubMed]
    [Google Scholar]
  22. Priest F. G., Goodfellow M., Todd C. ( 1988 ). A numerical classification of the genus Bacillus . . J Gen Microbiol 134, 1847–1882.[PubMed]
    [Google Scholar]
  23. Raj P. S., Ramaprasad E. V. V., Vaseef S., Sasikala Ch., Ramana Ch. V. ( 2013 ). Rhodobacter viridis sp. nov., a phototrophic bacterium isolated from mud of a stream. . Int J Syst Evol Microbiol 63, 181–186. [View Article] [PubMed]
    [Google Scholar]
  24. Romano I., Lama L., Nicolaus B., Gambacorta A., Giordano A. ( 2005 ). Bacillus saliphilus sp. nov., isolated from a mineral pool in Campania, Italy. . Int J Syst Evol Microbiol 55, 159–163. [View Article] [PubMed]
    [Google Scholar]
  25. Rüger H.-J., Richter G. ( 1979 ). Bacillus globisporus subsp. marinus subsp. nov.. Int J Syst Evol Microbiol 29, 196–203.
     [Google Scholar]
  26. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
     [Google Scholar]
  27. Schleifer K. H. ( 1985 ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18, 123–156. [View Article]
    [Google Scholar]
  28. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407–477.[PubMed]
    [Google Scholar]
  29. Seldin L., Dubnau D. ( 1985 ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. . Int J Syst Bacteriol 35, 151–154. [View Article]
    [Google Scholar]
  30. Shivaji S., Chaturvedi P., Suresh K., Reddy G. S. N., Dutt C. B. S., Wainwright M., Narlikar J. V., Bhargava P. M. ( 2006 ). Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes. . Int J Syst Evol Microbiol 56, 1465–1473. [View Article] [PubMed]
    [Google Scholar]
  31. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Evol Microbiol 44, 846–849.
     [Google Scholar]
  32. Subhash Y., Tushar L., Sasikala Ch., Ramana Ch. V. ( 2013 ). Falsirhodobacter halotolerans gen. nov., sp. nov., isolated from dry soils of a solar saltern. . Int J Syst Evol Microbiol 63, 2132–2137. [View Article] [PubMed]
    [Google Scholar]
  33. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  34. Täubel M., Kämpfer P., Buczolits S., Lubitz W., Busse H.-J. ( 2003 ). Bacillus barbaricus sp. nov., isolated from an experimental wall painting. . Int J Syst Evol Microbiol 53, 725–730. [View Article] [PubMed]
    [Google Scholar]
  35. Trüper H. G., Pfennig N. ( 1981 ). Isolation of members of the families Chromatiaceae and Chlorobiaceae . . In The Prokaryotes: a Handbook on Habitats, Isolation, and Identification of Bacteria, pp. 279–289. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . Berlin:: Springer;.
     [Google Scholar]
  36. Venkata Ramana V., Sasikala C., Veera Venkata Ramaprasad E., Venkata Ramana C. ( 2010 ). Description of Ectothiorhodospira salini sp. nov.. J Gen Appl Microbiol 56, 313–319. [View Article] [PubMed]
    [Google Scholar]
  37. Yoon J. H., Kim I. G., Kang K. H., Oh T. K., Park Y. H. ( 2004 ). Bacillus hwajinpoensis sp. nov. and an unnamed Bacillus genomospecies, novel members of Bacillus rRNA group 6 isolated from sea water of the East Sea and the Yellow Sea in Korea. . Int J Syst Evol Microbiol 54, 803–808. [View Article] [PubMed]
    [Google Scholar]
  38. Zhai L., Liao T., Xue Y., Ma Y. ( 2012 ). Bacillus daliensis sp. nov., an alkaliphilic, Gram-positive bacterium isolated from a soda lake. . Int J Syst Evol Microbiol 62, 949–953. [View Article] [PubMed]
    [Google Scholar]
  39. Zhang J., Wang J., Fang C., Song F., Xin Y., Qu L., Ding K. ( 2010 ). Bacillus oceanisediminis sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 60, 2924–2929. [View Article] [PubMed]
    [Google Scholar]
  40. Zhang J., Wang J., Song F., Fang C., Xin Y., Zhang Y. ( 2011 ). Bacillus nanhaiisediminis sp. nov., an alkalitolerant member of Bacillus rRNA group 6. . Int J Syst Evol Microbiol 61, 1078–1083. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.053504-0
Loading
Bacillus luteus sp. nov., isolated from soil
Int J Syst Evol Microbiol 64, 1580 (2014); https://doi.org/10.1099/ijs.0.053504-0
/content/journal/ijsem/10.1099/ijs.0.053504-0
/content/journal/ijsem/10.1099/ijs.0.053504-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error