RT Journal Article SR Electronic(1) A1 Hameed, Asif A1 Shahina, Mariyam A1 Lin, Shih-Yao A1 Lai, Wei-An A1 Hsu, Yi-Han A1 Liu, You-Cheng A1 Huang, Yu-Ming A1 Young, Chiu-ChungYR 2013 T1 Shimia biformata sp. nov., isolated from surface seawater, and emended description of the genus Shimia Choi and Cho 2006 JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_12 SP 4533 OP 4539 DO https://doi.org/10.1099/ijs.0.053553-0 PB Microbiology Society, SN 1466-5034, AB A novel, Gram-staining-negative, non-flagellated, oval or short-rod-shaped, strictly aerobic and non-spore-forming marine bacterium, designated strain CC-AMW-CT, was isolated from coastal surface seawater in Kending County, Taiwan. Cells of strain CC-AMW-CT displayed unusual morphology and formed colourless or beige colonies on marine agar. The isolate shared pairwise 16S rRNA gene sequence similarities of 97.2 and 97.1 % with Shimia marina BCRC 80068T and Shimia isoporae BCRC 80085T, respectively, and established a discrete phyletic lineage closely associated with the members of the genus Shimia . DNA–DNA hybridization values indicated <18.2 % genomic relatedness with species of the genus Shimia . The polar lipid profile of strain CC-AMW-CT comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids, four unidentified lipids and an unidentified phospholipid. The predominant fatty acids were C18 : 1ω7c and/or C18 : 1ω6c (summed feature 8; 75.5 %). The DNA G+C content was 61.2 mol%. The predominant respiratory quinone was ubiquinone Q-10 and the major polyamine was cadaverine. The chemotaxonomic evidence, including extraordinary amounts of C18 : 1ω7c and/or C18 : 1ω6c, major polar lipids, polyamine, quinone and DNA G+C contents of CC-AMW-CT, was in line with that of the members of the genus Shimia . Thus, strain CC-AMW-CT should be classified as a novel species of the genus Shimia , for which the name Shimia biformata sp. nov. is proposed. The type strain is CC-AMW-CT ( = JCM 18818T = BCRC 80548T). An emended description of the genus Shimia is also proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.053553-0