1887

Abstract

A novel thermophilic, facultatively anaerobic bacterium, strain skLN1, was isolated from the sediment of a freshwater lake in Japan. Cells of strain skLN1 were rod-shaped and Gram-stain-variable. A KOH lysis test suggested that the cell wall of the isolate has a Gram-positive structure. For aerobic growth, the optimum pH was pH 7.25–7.50 and the optimum temperature was 50–52 °C. The G+C content of the genomic DNA was 50.8 mol%. Nitrate was reduced to nitrite. Alicyclic fatty acids were not detected, and branched-chain fatty acids were major components in the cellular fatty acid profile. MK-7 was the predominant respiratory quinone. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolated strain was related most closely to CCUG 53762 (95 % similarity). This analysis also showed that the monophyly of the genus had been lost. On the basis of phylogenetic and phenotypic characterization, gen. nov., sp. nov. is proposed. The type strain of is skLN1 ( = NBRC 109614 = DSM 27172). It is also proposed that and should be reclassified to the genus as comb. nov. and comb. nov., respectively.

Funding
This study was supported by the:
  • JSPS KAKENHI (Award 22370005)
  • Institute for fermentation, Osaka
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.055814-0
2014-08-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/8/2770.html?itemId=/content/journal/ijsem/10.1099/ijs.0.055814-0&mimeType=html&fmt=ahah

References

  1. Bonjour F., Aragno M. ( 1984 ). Bacillus tusciae, a new species of thermoacidophilic, facultatively chemolithotrophic, hydrogen oxidizing sporeformer from a geothermal area. . Arch Microbiol 139, 397401. [View Article]
    [Google Scholar]
  2. da Costa M. S., Rainey F. A. ( 2009 ). Family II. Alicyclobacillaceae fam. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, p. 229. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  3. Glaeser S. P., Falsen E., Martin K., Kämpfer P. ( 2013 ). Alicyclobacillus consociatus sp. nov., isolated from a human clinical specimen. . Int J Syst Evol Microbiol 63, 36233627. [View Article] [PubMed]
    [Google Scholar]
  4. Gregersen T. ( 1978 ). Rapid method for distinction of gram-negative from gram-positive bacteria. . Eur J Appl Microbiol Biotechnol 5, 123127. [View Article]
    [Google Scholar]
  5. Imperio T., Viti C., Marri L. ( 2008 ). Alicyclobacillus pohliae sp. nov., a thermophilic, endospore-forming bacterium isolated from geothermal soil of the north-west slope of Mount Melbourne (Antarctica). . Int J Syst Evol Microbiol 58, 221225. [View Article] [PubMed]
    [Google Scholar]
  6. Katayama-Fujimura Y., Komatsu Y., Kuraishi H., Kaneko T. ( 1984 ). Estimation of DNA base composition by high-performance liquid chromatography of its nuclease PI hydrolysate. . Agric Biol Chem 48, 31693172. [View Article]
    [Google Scholar]
  7. Klenk H.-P., Lapidus A., Chertkov O., Copeland A., Del Rio T. G., Nolan M., Lucas S., Chen F., Tice H. & other authors ( 2011 ). Complete genome sequence of the thermophilic, hydrogen-oxidizing Bacillus tusciae type strain (T2) and reclassification in the new genus, Kyrpidia gen. nov. as Kyrpidia tusciae comb. nov. and emendation of the family Alicyclobacillaceae da Costa and Rainey, 2010. . Stand Genomic Sci 5, 121134. [View Article] [PubMed]
    [Google Scholar]
  8. Kojima H., Fukui M. ( 2010 ). Sulfuricella denitrificans gen. nov., sp. nov., a sulfur-oxidizing autotroph isolated from a freshwater lake. . Int J Syst Evol Microbiol 60, 28622866. [View Article] [PubMed]
    [Google Scholar]
  9. Kojima H., Iwata T., Fukui M. ( 2009 ). DNA-based analysis of planktonic methanotrophs in a stratified lake. . Freshw Biol 54, 15011509. [View Article]
    [Google Scholar]
  10. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in BacterialSsystematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . New York:: Wiley;.
    [Google Scholar]
  11. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. & other authors ( 2007 ). Clustal W and Clustal X version 2.0. . Bioinformatics 23, 29472948. [View Article] [PubMed]
    [Google Scholar]
  12. Muyzer G., Hottenträger S., Teske A., Wawer C. ( 1996 ). Denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA – a new molecular approach to analyze the genetic diversity of mixed microbial communities. . In Molecular Microbial Ecology Manual Edited by Akkermans A. D. L., Van Elsas J. D., De Brujin F. J. , pp. 123. Dordrecht:: Kluwer Academic;.
    [Google Scholar]
  13. Poulain S., Sergeant C., Simonoff M., Le Marrec C., Altmann S. ( 2008 ). Microbial investigations in Opalinus Clay, an argillaceous formation under evaluation as a potential host rock for a radioactive waste repository. . Geomicrobiol J 25, 240249. [View Article]
    [Google Scholar]
  14. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407477.[PubMed]
    [Google Scholar]
  15. Steven B., Chen M. Q., Greer C. W., Whyte L. G., Niederberger T. D. ( 2008 ). Tumebacillus permanentifrigoris gen. nov., sp. nov., an aerobic, spore-forming bacterium isolated from Canadian high Arctic permafrost. . Int J Syst Evol Microbiol 58, 14971501. [View Article] [PubMed]
    [Google Scholar]
  16. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  17. Widdel F., Bak F. ( 1992 ). Gram-negative mesophilic sulfate-reducing bacteria. . In The Prokaryotes, , 2nd edn., vol. 4, pp. 33523378. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. . New York:: Springer;. [View Article]
    [Google Scholar]
  18. Wisotzkey J. D., Jurtshuk P. Jr, Fox G. E., Deinhard G., Poralla K. ( 1992 ). Comparative sequence analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov.. Int J Syst Bacteriol 42, 263269. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.055814-0
Loading
/content/journal/ijsem/10.1099/ijs.0.055814-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error