1887

Abstract

A novel yeast species was found repeatedly and in high cell densities in underground-nesting stingless bees of the species and their provisions in northern Minas Gerais (Brazil). One additional strain was isolated from bee-collected pollen in Cuba. Phylogenetic analyses based on rRNA gene sequences (D1/D2 large subunit gene and internal transcribed spacer) indicated that the novel species belongs to the clade and is most closely related to (iter. nom. ) . Growth reactions on carbon and nitrogen sources were typical of those observed in related species of the clade. PCR-fingerprinting with mini- and microsatellite specific primers allowed the distinction of the novel species from , and a yet undescribed species represented by strain CBS 4353. On the basis of phylogenetic relationships, the novel species is assigned to the genus despite the failure to observe sexual reproduction after extensive mating tests. We propose the name f. a., sp. nov. (Mycobank MB 804285) and designate UFMG PST 09 ( = MUCL 53320 = CBS 12811) as the type strain.

Funding
This study was supported by the:
  • Conselho Nacional de Desenvolvimento Cientifico e Tecnológico
  • Fundação do Amparo a Pesquisa do Estado de Minas Gerais
  • Financiadora de Estudos e Projetos (Award 2084/07)
  • Natural Science and Engineering Research Council of Canada
  • Belgian Federal Science Policy (Award BCCM C4/10/017)
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2013-10-01
2024-04-18
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