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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_4

Description of gen. nov., sp. nov., isolated from flounder (), reclassification of four species of the genus as members of the genus gen. nov. and emended description of the genus Free

Abstract

A Gram-staining-negative, aerobic, rod-shaped bacterium, designated strain T202, was isolated from the gill of a cultured flounder (). Based on 16S rRNA gene sequence similarity, strain T202 was a member of the family and shared 93.32–96.58 % similarity with type strains of all members of the most closely related genus . Phylogenetically, the isolate shared a root with the type strains of four marine species, M-M1, TMA1, CBMAI 722 and JC2041. Optimal growth occurred in the presence of 2–4 % (w/v) NaCl, at pH 7.0–8.0 and at 28 °C. Ubiquinone 8 (Q-8) was the predominant respiratory quinone. The major fatty acids were C, summed feature 3 (Cω7 and/or Cω6), Cω9 and Cω8 The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain T202 was 37 mol%. On the basis of polyphasic analysis, especially the phylogenetic relationships and the lower DNA G+C content, strain T202 is considered to represent a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is T202 ( = JCM 18590 = DSM 26287 = KCTC 32144). Because M-M1, TMA1, CBMAI 722 and JC2041 formed a phylogenetic group together with strain T202 that was clearly separated from other known strains of , these four species are reclassified as members of the genus as comb. nov. (type strain M-M1 = KCTC 23343 = CCUG 60020), comb. nov. (type strain TMA1 = BCRC 17492 = JCM 13379 = DSM 19706), comb. nov. (type strain CBMAI 722 = LMG 22536) and comb. nov. (type strain JC2041 = IMSNU 14005 = KCTC 12041 = DSM 15355). The type species of the genus is gen. nov., comb. nov. An emended description of the genus is also proposed.

  • Published Online:
Funding
This study was supported by the:
  • International Science and Technology Cooperation Program of China (Award 2012DFG31990)
  • National High Technology Research and Development Program of China (863 program) (Award 2007AA09Z434)
© 2014 IUMS
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2014-04-01
2024-04-20
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Description of Thalassotalea piscium gen. nov., sp. nov., isolated from flounder (Paralichthys olivaceus), reclassification of four species of the genus Thalassomonas as members of the genus Thalassotalea gen. nov. and emended description of the genus Thalassomonas
Int J Syst Evol Microbiol 64, 1223 (2014); https://doi.org/10.1099/ijs.0.055913-0
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