@article{mbs:/content/journal/ijsem/10.1099/ijs.0.057265-0, author = "Amoozegar, Mohammad Ali and Bagheri, Maryam and Makhdoumi-Kakhki, Ali and Didari, Maryam and Schumann, Peter and Spröer, Cathrin and Sánchez-Porro, Cristina and Ventosa, Antonio", title = "Oceanobacillus limi sp. nov., a moderately halophilic bacterium from a salt lake", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2014", volume = "64", number = "Pt_4", pages = "1284-1289", doi = "https://doi.org/10.1099/ijs.0.057265-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.057265-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-stain-positive, endospore-forming, rod-shaped, strictly aerobic, moderately halophilic bacterium, designated strain H9BT, was isolated from a mud sample of the hypersaline lake Aran-Bidgol in Iran. Cells of strain H9BT were motile and produced colonies with a yellowish-grey pigment. Growth occurred between 2.5 and 10 % (w/v) NaCl and the isolate grew optimally at 7.5 % (w/v) NaCl. The optimum pH and temperature for growth of the strain were pH 7.0 and 35 °C, respectively, while it was able to grow over pH and temperature ranges of pH 6–10 and 25–45 °C, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H9BT is a member of the genus Oceanobacillus . The closest relative to this strain was Oceanobacillus profundus CL-MP28T with 97.1 % 16S rRNA gene sequences similarity. The level of DNA–DNA relatedness between the novel isolate and this phylogenetically related species was 17 %. The major cellular fatty acids of the isolate were anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C16 : 0. The polar lipid pattern of strain H9BT consisted of phosphatidylglycerol, diphosphatidylglycerol, four phospholipids and an aminolipid. It contained MK-7 as the predominant menaquinone and meso-diaminopimelic acid in the cell-wall peptidoglycan. The G+C content of the genomic DNA of this strain was 37.1 mol%. Phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data suggest that this strain represents a novel species of the genus Oceanobacillus , for which the name Oceanobacillus limi sp. nov. is proposed. The type strain of Oceanobacillus limi is strain H9BT ( = IBRC-M 10780T = KCTC 13823T = CECT 7997T).", }