RT Journal Article SR Electronic(1) A1 Zhou, Liguang A1 Liu, Xiaoli A1 Dong, XiuzhuYR 2014 T1 Methanospirillum psychrodurum sp. nov., isolated from wetland soil JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_2 SP 638 OP 641 DO https://doi.org/10.1099/ijs.0.057299-0 PB Microbiology Society, SN 1466-5034, AB A psychrotolerant methanogenic strain, X-18T, was isolated from the soil of the Madoi wetland at Qinghai, Tibetan plateau, China. Cells were wavy rods (11–62 µm long) with blunt tapered ends and Gram-stain-negative. Strain X-18T grew strictly anaerobically and produced methane exclusively from H2/CO2. Growth occurred in the temperature range of 4–32 °C and optimally at 25 °C. Growth pH ranged from 6.5 to 8.0 and the optimum was 7.0. The G+C content of the genomic DNA of strain X-18T was 44.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences and the alpha subunit of methyl-coenzyme M reductase indicated that strain X-18T was affiliated to the genus Methanospirillum and was most closely related to Methanospirillum lacunae Ki8-1T, with 96.3 % 16S rRNA gene sequence similarity. However, strain X-18T could be distinguished from the existing species of the genus Methanospirillum by its lower growth temperature and obligate hydrogenotrophic methanogenesis. On the basis of phenotypic characteristics and phylogenetic analysis, strain X-18T represents a novel species of the genus Methanospirillum , for which the name Methanospirillum psychrodurum sp. nov. is proposed and strain X-18T is assigned as the type strain ( = CGMCC 1.5186T = JCM 19216T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.057299-0