RT Journal Article SR Electronic(1) A1 Kim, Soo-Jin A1 Lim, Jun-Muk A1 Ahn, Jae-Hyung A1 Weon, Hang-Yeon A1 Hamada, Moriyuki A1 Suzuki, Ken-ichiro A1 Ahn, Tae-Young A1 Kwon, Soon-WoYR 2014 T1 Description of Galbitalea soli gen. nov., sp. nov., and Frondihabitans sucicola sp. nov. JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_2 SP 572 OP 578 DO https://doi.org/10.1099/ijs.0.058339-0 PB Microbiology Society, SN 1466-5034, AB Bacterial strains KIS82-1T and GRS42T were isolated from soil and from sap of Acer mono, respectively, in the Republic of Korea. Both strains were aerobic, Gram-stain-positive, mesophilic, rod-shaped and motile. Phylogenetically, both strains belonged to the family Microbacteriaceae of the phylum Actinobacteria . The 16S rRNA gene sequence of strain KIS82-1T showed the highest similarity to those of Frondihabitans peucedani RS-15T (97.6 %), Frigoribacterium mesophilum MSL-08T (97.2 %) and Labedella gwakjiensis KSW2-17T (97.0 %), while strain GRS42T showed the highest 16S rRNA gene sequence similarity to Frondihabitans peucedani RS-15T (98.7 %), Frondihabitans cladoniiphilus CafT13T (98.4 %), Frondihabitans australicus E1HC-02T (98.2 %) and Frigoribacterium faeni 801T (97.3 %). The 16S rRNA gene sequence similarity between GRS42T and KIS82-1T was 97.0 %. Phylogenetic trees indicated that strain GRS42T was firmly grouped into the genus Frondihabitans , while strain KIS82-1T did not show a clear affiliation to any genus within the family Microbacteriaceae . Strain KIS82-1T showed type B1β peptidoglycan with 2,4-diamino-l-butyric acid as the diamino acid. It had MK-11, MK-10 and MK-12 as respiratory quinones, anteiso-C15 : 0, iso-C16 : 0 and iso-C14 : 0 as major cellular fatty acids and diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid as predominant polar lipids. The peptidoglycan of strain GRS42T was of type B2β with d-ornithine as the diamino acid. The strain contained MK-8, MK-9 and MK-7 as respiratory quinones, summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c) as major cellular fatty acid and diphosphatidylglycerol, phosphatidylglycerol and three unknown glycolipids as predominant polar lipids. Strain GRS42T revealed low DNA–DNA hybridization (<50 % relatedness) with closely related strains. Based on the data obtained in the present polyphasic taxonomic study, we propose that strain KIS82-1T represents a novel genus and species and that strain GRS42T represents a novel species in the family Microbacteriaceae . The genus Galbitalea gen. nov. is proposed, with strain KIS82-1T ( = KACC 15520T = NBRC 108727T) as the type strain of the type species, Galbitalea soli sp. nov. Strain GRS42T ( = KACC 15521T = NBRC 108728T) is proposed as the type strain of Frondihabitans sucicola sp. nov., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.058339-0