1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_8

sp. nov., an acidophilic bacterium isolated from cherry bark Free

Abstract

A novel acidobacterial strain, Sakura1, was isolated from pieces of cherry bark. Cells of strain Sakura1 were non-motile, rod-shaped and stained Gram-negative. This strictly aerobic isolate was mesophilic but was able to grow at temperatures as low as 10 °C. Colonies were pink due to production of carotenoids, and its pigmentation was more pronounced in cells grown at lower temperature. This strain had endoglucanase activity. The main respiratory quinone was menaquinone-8, and major cellular fatty acids were iso-C, Cω7 and C. Phylogenetic analysis of 16S rRNA gene sequences revealed that the strain was closely related to species of the genus in subdivision 1 of the phylum . The closest relative was OB1010 (97.1 % 16S rRNA gene sequence similarity). While common characteristics were found among the isolate and species of the genus there were obvious differences between them such as their cell morphology, cellulolytic activity, and tolerance to low temperature and NaCl concentration. Based on phylogenetic and phenotypic findings, a novel species is proposed in the genus with the name sp. nov. The type strain is Sakura1 ( = NBRC 107139 = DSM 23641).

  • Published Online:
© 2014 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.058636-0
2014-08-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/8/2781.html?itemId=/content/journal/ijsem/10.1099/ijs.0.058636-0&mimeType=html&fmt=ahah

References

  1. Barns S. M., Takala S. L., Kuske C. R. ( 1999 ). Wide distribution and diversity of members of the bacterial kingdom Acidobacterium in the environment. . Appl Environ Microbiol 65, 1731–1737.[PubMed]
    [Google Scholar]
  2. Barns S. M., Cain E. C., Sommerville L., Kuske C. R. ( 2007 ). Acidobacteria phylum sequences in uranium-contaminated subsurface sediments greatly expand the known diversity within the phylum. . Appl Environ Microbiol 73, 3113–3116. [View Article] [PubMed]
    [Google Scholar]
  3. Bryant D. A., Costas A. M. G., Maresca J. A., Chew A. G. M., Klatt C. G., Bateson M. M., Tallon L. J., Hostetler J., Nelson W. C. & other authors ( 2007 ). Candidatus Chloracidobacterium thermophilum: an aerobic phototrophic acidobacterium. . Science 317, 523–526. [View Article] [PubMed]
    [Google Scholar]
  4. Dedysh S. N., Kulichevskaya I. S., Serkebaeva Y. M., Mityaeva M. A., Sorokin V. V., Suzina N. E., Rijpstra W. I. C., Damsté J. S. S. ( 2012 ). Bryocella elongata gen. nov., sp. nov., a member of subdivision 1 of the Acidobacteria isolated from a methanotrophic enrichment culture, and emended description of Edaphobacter aggregans Koch et al. 2008. . Int J Syst Evol Microbiol 62, 654–664. [View Article] [PubMed]
    [Google Scholar]
  5. Eichorst S. A., Breznak J. A., Schmidt T. M. ( 2007 ). Isolation and characterization of soil bacteria that define Terriglobus gen. nov., in the phylum Acidobacteria . . Appl Environ Microbiol 73, 2708–2717. [View Article] [PubMed]
    [Google Scholar]
  6. Hallberg K. B., Coupland K., Kimura S., Johnson D. B. ( 2006 ). Macroscopic streamer growths in acidic, metal-rich mine waters in North Wales consist of novel and remarkably simple bacterial communities. . Appl Environ Microbiol 72, 2022–2030. [View Article] [PubMed]
    [Google Scholar]
  7. Hugenholtz P., Goebel B. M., Pace N. R. ( 1998 ). Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. . J Bacteriol 180, 4765–4774.[PubMed]
    [Google Scholar]
  8. Janssen P. H. ( 2006 ). Identifying the dominant soil bacterial taxa in libraries of 16S rRNA and 16S rRNA genes. . Appl Environ Microbiol 72, 1719–1728. [View Article] [PubMed]
    [Google Scholar]
  9. Kamagata Y., Mikami E. ( 1991 ). Isolation and characterization of a novel thermophilic Methanosaeta strain. . Int J Syst Bacteriol 41, 191–196. [View Article]
    [Google Scholar]
  10. Kishimoto N., Kosako Y., Tano T. ( 1991 ). Acidobacterium capsulatum gen. nov., sp. nov.: an acidophilic chemoorganotrophic bacterium containing menaquinone from acidic mineral environment. . Curr Microbiol 22, 1–7. [View Article]
    [Google Scholar]
  11. Koch I. H., Gich F., Dunfield P. F., Overmann J. ( 2008 ). Edaphobacter modestus gen. nov., sp. nov., and Edaphobacter aggregans sp. nov., acidobacteria isolated from alpine and forest soils. . Int J Syst Evol Microbiol 58, 1114–1122. [View Article] [PubMed]
    [Google Scholar]
  12. Kulichevskaya I. S., Kostina L. A., Valásková V., Rijpstra W. I. C., Damsté J. S. S., de Boer W., Dedysh S. N. ( 2012 ). Acidicapsa borealis gen. nov., sp. nov. and Acidicapsa ligni sp. nov., subdivision 1 Acidobacteria from Sphagnum peat and decaying wood. . Int J Syst Evol Microbiol 62, 1512–1520. [View Article] [PubMed]
    [Google Scholar]
  13. Liesack W., Bak F., Kreft J. U., Stackebrandt E. ( 1994 ). Holophaga foetida gen. nov., sp. nov., a new, homoacetogenic bacterium degrading methoxylated aromatic compounds. . Arch Microbiol 162, 85–90.[PubMed]
    [Google Scholar]
  14. Männistö M. K., Rawat S., Starovoytov V., Häggblom M. M. ( 2012 ). Granulicella arctica sp. nov., Granulicella mallensis sp. nov., Granulicella tundricola sp. nov. and Granulicella sapmiensis sp. nov., novel acidobacteria from tundra soil. . Int J Syst Evol Microbiol 62, 2097–2106. [View Article] [PubMed]
    [Google Scholar]
  15. Marichamy S., Mattiasson B. ( 2005 ). Rapid production of cellulase-free xylanases by solventogenic Clostridia from rumen. . Enzyme Microb Technol 37, 497–504. [View Article]
    [Google Scholar]
  16. Pankratov T. A., Dedysh S. N. ( 2010 ). Granulicella paludicola gen. nov., sp. nov., Granulicella pectinivorans sp. nov., Granulicella aggregans sp. nov. and Granulicella rosea sp. nov., acidophilic, polymer-degrading acidobacteria from Sphagnum peat bogs. . Int J Syst Evol Microbiol 60, 2951–2959. [View Article] [PubMed]
    [Google Scholar]
  17. Pankratov T. A., Kirsanova L. A., Kaparullina E. N., Kevbrin V. V., Dedysh S. N. ( 2012 ). Telmatobacter bradus gen. nov., sp. nov., a cellulolytic facultative anaerobe from subdivision 1 of the Acidobacteria, and emended description of Acidobacterium capsulatum Kishimoto et al. 1991. . Int J Syst Evol Microbiol 62, 430–437. [View Article] [PubMed]
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  19. Selenska-Pobell S., Flemming K., Tzvetkova T., Raff J., Schnorpfeil M., Geißler A. ( 2003 ). Bacterial communities in uranium mining waste piles and their interaction with heavy metals. . In Uranium in the Aquatic Environment: proceedings of the International Conference [on] Uranium Mining and Hydrogeology III and the International Mine Water Association Symposium, pp. 455–464. Edited by Merkel B. J., Planer-Friedrich B., Wolkersdorfer C. . New York:: Springer;.
     [Google Scholar]
  20. Shintani T., Liu W. T., Hanada S., Kamagata Y., Miyaoka S., Suzuki T., Nakamura K. ( 2000 ). Micropruina glycogenica gen. nov., sp. nov., a new Gram-positive glycogen-accumulating bacterium isolated from activated sludge. . Int J Syst Evol Microbiol 50, 201–207. [View Article] [PubMed]
    [Google Scholar]
  21. Somogyi M. ( 1952 ). Notes on sugar determination. . J Biol Chem 195, 19–23.[PubMed]
    [Google Scholar]
  22. Takaichi S., Maoka T., Takasaki K., Hanada S. ( 2010 ). Carotenoids of Gemmatimonas aurantiaca (Gemmatimonadetes): identification of a novel carotenoid, deoxyoscillol 2-rhamnoside, and proposed biosynthetic pathway of oscillol 2,2′-dirhamnoside. . Microbiology 156, 757–763. [View Article] [PubMed]
    [Google Scholar]
  23. Tamaki H., Hanada S., Kamagata Y., Nakamura K., Nomura N., Nakano K., Matsumura M. ( 2003 ). Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments. . Int J Syst Evol Microbiol 53, 519–526. [View Article] [PubMed]
    [Google Scholar]
  24. Zhang H., Hanada S., Shigematsu T., Shibuya K., Kamagata Y., Kanagawa T., Kurane R. ( 2000 ). Burkholderia kururiensis sp. nov., a trichloroethylene (TCE)-degrading bacterium isolated from an aquifer polluted with TCE. . Int J Syst Evol Microbiol 50, 743–749. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.058636-0
Loading
Granulicellacerasi sp. nov., an acidophilic bacterium isolated from cherry bark
Int J Syst Evol Microbiol 64, 2781 (2014); https://doi.org/10.1099/ijs.0.058636-0
/content/journal/ijsem/10.1099/ijs.0.058636-0
/content/journal/ijsem/10.1099/ijs.0.058636-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error