RT Journal Article SR Electronic(1) A1 Parkinson, Neil A1 DeVos, Paul A1 Pirhonen, Minna A1 Elphinstone, JohnYR 2014 T1 Dickeya aquatica sp. nov., isolated from waterways JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_7 SP 2264 OP 2266 DO https://doi.org/10.1099/ijs.0.058693-0 PB Microbiology Society, SN 1466-5034, AB Pectinolytic Gram-negative bacteria were isolated from different waterways in the UK and Finland. Three strains (174/2T, 181/2 and Dw054) had the same 16S rRNA gene sequences which shared 99 % sequence similarity to species of the genus Dickeya , and a phylogeny of related genera confirmed attribution to this genus. Fatty acid profile analysis of all three strains found a high proportion of C16 : 1ω7c/C16 : 1ω7c and C16 : 0 fatty acids, and library profile searches found closest matches to Dickeya chrysanthemi . Production of a concatenated phylogeny using six loci, recA, gapA, atpD, gyrB, infB and rpoB, provided a high-resolution phylogeny which placed strains 174/2T and 181/2 as a distinct clade, separated from the other species of the genus Dickeya by a relatively long branch-length. DNA–DNA hybridization analysis with a limited number of reference species also supported the distinctiveness of strains 174/2T and 181/2 within the genus Dickeya . All three strains could be phenotypically distinguished from other species of the genus by fermentation of melibiose and raffinose but not d-arabinose or mannitol. The name Dickeya aquatica sp. nov. is proposed for the new taxon; the type strain is 174/2T ( = NCPPB 4580T = LMG 27354T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.058693-0