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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_7

sp. nov., an obligate anaerobic bacteria isolated from human faeces Free

Abstract

Two coccoid, non-motile, obligately anaerobic, Gram-stain-negative bacteria, occurring singly or in pairs, or as short chains, with a mean size of 1.4–2.5 µm were isolated from the faeces of two healthy human volunteers, aged 26 and 56 years, and were designated NMBHI-10 and BLPYG-7, respectively. Both the strains were affiliated to the sub-branch of the class as revealed by 16S rRNA gene sequence analysis. The isolates NMBHI-10 and BLPYG-7 showed 99.1 and 99.2 % 16S rRNA gene sequence similarity, respectively, with JCM 1772. DNA–DNA hybridization and phenotypic analysis showed that both the strains were distinct from their closest relative, JCM 1772 (42 and 53 % DNA–DNA relatedness with NMBHI-10 and BLPYG-7, respectively), but belong to the same species (DNA–DNA relatedness of 80.9 % between the isolates). According to DNA–DNA hybridization results, the coccoid strains belong to the same genospecies, and neither is related to any of the recognized species of the genus . Strains NMBHI-10 and BLPYG-7 grew in PYG broth at temperatures of between 15 and 40 °C (optimum 37 °C), but not at 45 °C. The strains utilized a range of carbohydrates as sources of carbon and energy including glucose, lactose, cellobiose, rhamnose, galactose and sucrose. Glucose fermentation resulted in the formation of volatile fatty acids, mainly caproic acid and organic acids such as succinic acid. Phylogenetic analysis, specific phenotypic characteristics and/or DNA G+C content also differentiated the strains from each other and from their closest relatives. The DNA GC contents of strains NMBHI-10 and BLPYG-7 are 57.7 and 54.9 mol%, respectively. The major fatty acids were 12 : 0 FAME and 17 : 0 CYC FAME. On the basis of these data, we conclude that strains NMBHI-10 and BLPYG-7 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NMBHI-10 ( = DSM 25563 = MCC 2481).

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2014-07-01
2024-04-19
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Megasphaera indica sp. nov., an obligate anaerobic bacteria isolated from human faeces
Int J Syst Evol Microbiol 64, 2250 (2014); https://doi.org/10.1099/ijs.0.059816-0
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