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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_1

sp. nov., a bioflocculant-producing species isolated from soil under a peach tree Free

Abstract

A Gram-stain-positive, aerobic or facultatively anaerobic, rod-shaped, non-motile, endospore-forming bacterium, strain A9, was isolated in 1996 from a soil sample collected under a peach tree in Qingnian Park in Shenyang, PR China, and its taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belonged to the genus , and was most closely related to the type strain of with a 16S rRNA gene sequence similarity of 96.7 % and a DNA–DNA relatedness value of 51.6 %. The major polar lipids of strain A9 were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant menaquinone was MK-7 and the major cellular fatty acids were anteiso-C, C and iso-C. The DNA G+C content was 51.9 mol%. Based on these results, it is concluded that strain A9 represents a novel species of the genus , for which the name sp. nov. is proposed, with A9 ( = JCM 19307 = CGMCC 2040) as the type strain.

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award N120601003 and 51278090, 51408103)
  • Major Science and Technology Program for Water Pollution Control and Treatment (Award 2012ZX07202-004-06, 013ZX07202-010-05)
  • Fundamental Research Funds for the Central Universities of China
© 2015 IUMS
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2015-01-01
2024-04-19
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References

  1. Ash C., Priest F. G., Collins M. D. ( 1993 ). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. . Antonie van Leeuwenhoek 64, 253–260. [View Article] [PubMed]
    [Google Scholar]
  2. Ash C., Priest F. G., Collins M. D. ( 1994 ). Paenibacillus gen. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 51. . Int J Syst Bacteriol 44, 852–853. [View Article]
    [Google Scholar]
  3. Berge O., Guinebretière M. H., Achouak W., Normand P., Heulin T. ( 2002 ). Paenibacillus graminis sp. nov. and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and food. . Int J Syst Evol Microbiol 52, 607–616.[PubMed]
    [Google Scholar]
  4. Chou J. H., Chou Y. J., Lin K. Y., Sheu S. Y., Sheu D. S., Arun A. B., Young C. C., Chen W. M. ( 2007 ). Paenibacillus fonticola sp. nov., isolated from a warm spring. . Int J Syst Evol Microbiol 57, 1346–1350. [View Article] [PubMed]
    [Google Scholar]
  5. Chung Y. R., Kim C. H., Hwang I., Chun J. ( 2000 ). Paenibacillus koreensis sp. nov., a new species that produces an iturin-like antifungal compound. . Int J Syst Evol Microbiol 50, 1495–1500. [View Article] [PubMed]
    [Google Scholar]
  6. Cole R. M., Popkin T. J. ( 1981 ). Electron microscopy. . In Manual of Methods for General Bacteriology, pp. 34–51. Edited by Gerhardt P., Murray R. G. E., Costilaw R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  7. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
     [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224–229. [View Article]
    [Google Scholar]
  9. Hong Y. Y., Ma Y. C., Zhou Y. G., Gao F., Liu H. C., Chen S. F. ( 2009 ). Paenibacillus sonchi sp. nov., a nitrogen-fixing species isolated from the rhizosphere of Sonchus oleraceus . . Int J Syst Evol Microbiol 59, 2656–2661. [View Article] [PubMed]
    [Google Scholar]
  10. Hucker G. J., Conn H. J. ( 1923 ). Method of Gram staining. . N Y State Agric Exp Stn Tech Bull 93, 3–37.
     [Google Scholar]
  11. Jin H. J., Lv J., Chen S. F. ( 2011 ). Paenibacillus sophorae sp. nov., a nitrogen-fixing species isolated from the rhizosphere of Sophora japonica . . Int J Syst Evol Microbiol 61, 767–771. [View Article] [PubMed]
    [Google Scholar]
  12. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. H. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  13. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 989–1005. [View Article]
    [Google Scholar]
  14. Kämpfer P., Rosselló-Mora R., Falsen E., Busse H. J., Tindall B. J. ( 2006 ). Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov.. Int J Syst Evol Microbiol 56, 781–786. [View Article] [PubMed]
    [Google Scholar]
  15. Khianngam S., Tanasupawat S., Akaracharanya A., Kim K. K., Lee K. C., Lee J. S. ( 2011 ). Paenibacillus xylanisolvens sp. nov., a xylan-degrading bacterium from soil. . Int J Syst Evol Microbiol 61, 160–164. [View Article] [PubMed]
    [Google Scholar]
  16. Kim D. S., Bae C. Y., Jeon J. J., Chun S. J., Oh H. W., Hong S. G., Baek K. S., Moon E. Y., Bae K. S. ( 2004 ). Paenibacillus elgii sp. nov., with broad antimicrobial activity. . Int J Syst Evol Microbiol 54, 2031–2035. [View Article] [PubMed]
    [Google Scholar]
  17. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  18. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  19. Kumar S., Nei M., Dudley J., Tamura K. ( 2008 ). mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. . Brief Bioinform 9, 299–306. [View Article] [PubMed]
    [Google Scholar]
  20. Minnikin D. E., Odonnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  21. Nelson D. M., Glawe A. J., Labeda D. P., Cann I. K. O., Mackie R. I. ( 2009 ). Paenibacillus tundrae sp. nov. and Paenibacillus xylanexedens sp. nov., psychrotolerant, xylan-degrading bacteria from Alaskan tundra. . Int J Syst Evol Microbiol 59, 1708–1714. [View Article] [PubMed]
    [Google Scholar]
  22. Osman S., Satomi M., Venkateswaran K. ( 2006 ). Paenibacillus pasadenensis sp. nov. and Paenibacillus barengoltzii sp. nov., isolated from a spacecraft assembly facility. . Int J Syst Evol Microbiol 56, 1509–1514. [View Article] [PubMed]
    [Google Scholar]
  23. Ren N., Xing D., Rittmann B. E., Zhao L., Xie T., Zhao X. ( 2007 ). Microbial community structure of ethanol type fermentation in bio-hydrogen production. . Environ Microbiol 9, 1112–1125. [View Article] [PubMed]
    [Google Scholar]
  24. Roux V., Raoult D. ( 2004 ). Paenibacillus massiliensis sp. nov., Paenibacillus sanguinis sp. nov. and Paenibacillus timonensis sp. nov., isolated from blood cultures. . Int J Syst Evol Microbiol 54, 1049–1054. [View Article] [PubMed]
    [Google Scholar]
  25. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical note 101. Newark, DE: MIDI, Inc..
    [Google Scholar]
  26. Scheldeman P., Goossens K., Rodriguez-Diaz M., Pil A., Goris J., Herman L., De Vos P., Logan N. A., Heyndrickx M. ( 2004 ). Paenibacillus lactis sp. nov., isolated from raw and heat-treated milk. . Int J Syst Evol Microbiol 54, 885–891. [View Article] [PubMed]
    [Google Scholar]
  27. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125–128. [View Article]
    [Google Scholar]
  28. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 4876–4882. [View Article] [PubMed]
    [Google Scholar]
  29. Velázquez E., de Miguel T., Poza M., Rivas R., Rosselló-Mora R., Villa T. G. ( 2004 ). Paenibacillus favisporus sp. nov., a xylanolytic bacterium isolated from cow faeces. . Int J Syst Evol Microbiol 54, 59–64. [View Article] [PubMed]
    [Google Scholar]
  30. Wang L., Baek S. H., Cui Y., Lee H. G., Lee S. T. ( 2012 ). Paenibacillus sediminis sp. nov., a xylanolytic bacterium isolated from a tidal flat. . Int J Syst Evol Microbiol 62, 1284–1288. [View Article] [PubMed]
    [Google Scholar]
  31. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989 ). The analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16, 176–178 [View Article]
    [Google Scholar]
  32. Wu X., Fang H., Qian C., Wen Y., Shen X., Li O., Gao H. ( 2011 ). Paenibacillus tianmuensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 61, 1133–1137. [View Article] [PubMed]
    [Google Scholar]
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Paenibacillus shenyangensis sp. nov., a bioflocculant-producing species isolated from soil under a peach tree
Int J Syst Evol Microbiol 65, 220 (2015); https://doi.org/10.1099/ijs.0.060483-0
/content/journal/ijsem/10.1099/ijs.0.060483-0
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