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Volume 64, Issue Pt_10

gen. nov., sp. nov., a Gram-negative, aerobic, filamentous bacterium of a novel proteobacterial lineage, and description of fam. nov., ord. nov. and classis nov. Open Access

Abstract

A phylogenetically novel proteobacterium, strain Shr3, was isolated from sand gravels collected from the eastern margin of the Sahara Desert. The isolation strategy targeted bacteria filterable through 0.2-µm-pore-size filters. Strain Shr3 was determined to be a Gram-negative, aerobic, non-motile, filamentous bacterium. Oxidase and catalase reactions were positive. Strain Shr3 showed growth on R2A medium, but poor or no growth on nutrient agar, trypticase soy agar and standard method agar. The major isoprenoid quinone was menaquinone-7. The dominant cellular fatty acids detected were Cω5 and C, and the primary hydroxy acid present was C 3-OH. The DNA G+C content was 54.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Shr3 was affiliated with an uncultivated lineage of the phylum ; the nearest known type strain, with 83 % sequence similarity, was DSM 12838 in the class . The isolate and closely related environmental clones formed a novel class-level clade in the phylum with high bootstrap support (96–99 %). Based on these results, the novel class classis nov. in the phylum and the novel genus and species gen. nov., sp. nov. are proposed for strain Shr3, the first cultivated representative of the . The type strain of is Shr3 ( = JCM 16864 = NCIMB 14846). We also propose the subordinate taxa ord. nov. and fam. nov. in the class .

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Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award 23570117)
© 2014 IUMS
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2014-10-01
2024-04-18
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Oligoflexus tunisiensis gen. nov., sp. nov., a Gram-negative, aerobic, filamentous bacterium of a novel proteobacterial lineage, and description of Oligoflexaceae fam. nov., Oligoflexales ord. nov. and Oligoflexia classis nov.
Int J Syst Evol Microbiol 64, 3353 (2014); https://doi.org/10.1099/ijs.0.060798-0
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