RT Journal Article SR Electronic(1) A1 Takeuchi, Mio A1 Kamagata, Yoichi A1 Oshima, Kenshiro A1 Hanada, Satoshi A1 Tamaki, Hideyuki A1 Marumo, Katsumi A1 Maeda, Hiroto A1 Nedachi, Munetomo A1 Hattori, Masahira A1 Iwasaki, Wataru A1 Sakata, SusumuYR 2014 T1 Methylocaldum marinum sp. nov., a thermotolerant, methane-oxidizing bacterium isolated from marine sediments, and emended description of the genus Methylocaldum JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_9 SP 3240 OP 3246 DO https://doi.org/10.1099/ijs.0.063503-0 PB Microbiology Society, SN 1466-5034, AB An aerobic, methane-oxidizing bacterium (strain S8T) was isolated from marine sediments in Kagoshima Bay, Japan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain is closely related to members of the genus Methylocaldum (97.6–97.9 % similarity) within the class Gammaproteobacteria . Strain S8T was a Gram-staining-negative, non-motile, coccoid or short rod-shaped organism. The temperature range for growth of strain S8T was 20–47 °C (optimum growth at 36 °C). It required NaCl (>0.5 %), tolerated up to 5 % NaCl and utilized methane and methanol. The major cellular fatty acid and major respiratory quinone were C16 : 0 and 18-methylene ubiquinone 8, respectively. The DNA G+C content was 59.7 mol%. Strain S8T possessed mmoX, which encodes soluble methane monooxygenase, as well as pmoA, which encodes the particulate methane monooxygenase. On the basis of this morphological, physiological, biochemical and genetic information, the first marine species in the genus Methylocaldum is proposed, with the name Methylocaldum marinum sp. nov. The type strain is S8T ( = NBRC 109686T = DSM 27392T). An emended description of the genus Methylocaldum is also provided., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.063503-0