RT Journal Article SR Electronic(1) A1 Kim, Young-Ok A1 Park, Sooyeon A1 Nam, Bo-Hye A1 Park, Ji-Min A1 Kim, Dong-Gyun A1 Yoon, Jung-HoonYR 2014 T1 Litoreibacterascidiaceicola sp. nov., isolated from the golden sea squirt Halocynthiaaurantium JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_8 SP 2545 OP 2550 DO https://doi.org/10.1099/ijs.0.064196-0 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated RSS4-C1T, was isolated from a golden sea squirt (Halocynthia aurantium) collected from the East Sea, South Korea. The novel strain grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 1.0–3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain RSS4-C1T fell within the clade comprising the type strains of species of the genus Litoreibacter . Strain RSS4-C1T exhibited the highest 16S rRNA gene sequence similarity (99.6 %) to the type strain of Litoreibacter albidus and sequence similarities of 96.5–98.5 % to type strains of other recognized species of the genus Litoreibacter . Strain RSS4-C1T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c and 11-methyl-C18 : 1ω7c as the major fatty acids. The major polar lipids of strain RSS4-C1T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C content of strain RSS4-C1T was 58.0 mol% and its DNA–DNA relatedness values with type strains of four species of the genus Litoreibacter were 21–34 %. The differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain RSS4-C1T is distinct from other species of the genus Litoreibacter . On the basis of the data presented, strain RSS4-C1T is considered to represent a novel species of the genus Litoreibacter , for which the name Litoreibacter ascidiaceicola sp. nov. is proposed. The type strain is RSS4-C1T ( = KCTC 42050T = CECT 8539T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.064196-0