@article{mbs:/content/journal/ijsem/10.1099/ijs.0.066662-0, author = "Zhou, Shungui and Yang, Guiqin and Lu, Qin and Wu, Min", title = "Geobactersoli sp. nov., a dissimilatory Fe(III)-reducing bacterium isolated from forest soil ", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2014", volume = "64", number = "Pt_11", pages = "3786-3791", doi = "https://doi.org/10.1099/ijs.0.066662-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.066662-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel Fe(III)-reducing bacterium, designated GSS01T, was isolated from a forest soil sample using a liquid medium containing acetate and ferrihydrite as electron donor and electron acceptor, respectively. Cells of strain GSS01T were strictly anaerobic, Gram-stain-negative, motile, non-spore-forming and slightly curved rod-shaped. Growth occurred at 16–40 °C and optimally at 30 °C. The DNA G+C content was 60.9 mol%. The major respiratory quinone was MK-8. The major fatty acids were C16 : 0, C18 : 0 and C16 : 1ω7c/C16 : 1ω6c. Strain GSS01T was able to grow with ferrihydrite, Fe(III) citrate, Mn(IV), sulfur, nitrate or anthraquinone-2,6-disulfonate, but not with fumarate, as sole electron acceptor when acetate was the sole electron donor. The isolate was able to utilize acetate, ethanol, glucose, lactate, butyrate, pyruvate, benzoate, benzaldehyde, m-cresol and phenol but not toluene, p-cresol, propionate, malate or succinate as sole electron donor when ferrihydrite was the sole electron acceptor. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain GSS01T was most closely related to Geobacter sulfurreducens PCAT (98.3 % sequence similarity) and exhibited low similarities (94.9–91.8 %) to the type strains of other species of the genus Geobacter . The DNA–DNA relatedness between strain GSS01T and G. sulfurreducens PCAT was 41.4±1.1 %. On the basis of phylogenetic analysis, phenotypic characterization and physiological tests, strain GSS01T is believed to represent a novel species of the genus Geobacter , and the name Geobacter soli sp. nov. is proposed. The type strain is GSS01T ( = KCTC 4545T = MCCC 1K00269T).", }