RT Journal Article SR Electronic(1) A1 Junghare, Madan A1 Schink, BernhardYR 2015 T1 Desulfoprunum benzoelyticum gen. nov., sp. nov., a Gram-stain-negative, benzoate-degrading, sulfate-reducing bacterium isolated from a wastewater treatment plant JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_1 SP 77 OP 84 DO https://doi.org/10.1099/ijs.0.066761-0 PB Microbiology Society, SN 1466-5034, AB A strictly anaerobic, mesophilic, sulfate-reducing bacterium, strain KoBa311T, isolated from the wastewater treatment plant at Konstanz, Germany, was characterized phenotypically and phylogenetically. Cells were Gram-stain-negative, non-motile, oval to short rods, 3–5 µm long and 0.8–1.0 µm wide with rounded ends, dividing by binary fission and occurring singly or in pairs. The strain grew optimally in freshwater medium and the optimum temperature was 30 °C. Strain KoBa311T showed optimum growth at pH 7.3−7.6. Organic electron donors were oxidized completely to carbon dioxide concomitant with sulfate reduction to sulfide. At excess substrate supply, substrates were oxidized incompletely and acetate (mainly) and/or propionate accumulated. The strain utilized short-chain fatty acids, alcohols (except methanol) and benzoate. Sulfate and DMSO were used as terminal electron acceptors for growth. The genomic DNA G+C content was 52.3 mol% and the respiratory quinone was menaquinone MK-5 (V-H2). The major fatty acids were C16 : 0, C16 : 1ω7c/ω6c and C18 : 1ω7c. Phylogenetic analysis based on 16S rRNA gene sequences placed strain KoBa311T within the family Desulfobulbaceae in the class Deltaproteobacteria . Its closest related bacterial species on the basis of the distance matrix were Desulfobacterium catecholicum DSM 3882T (93.0 % similarity), Desulfocapsa thiozymogenes (93.1 %), Desulforhopalus singaporensis (92.9 %), Desulfopila aestuarii (92.4 %), Desulfopila inferna JS_SRB250LacT (92.3 %) and Desulfofustis glycolicus (92.3 %). On the basis of phylogenetic, physiological and chemotaxonomic characteristics, strain KoBa311T was distinct from any related type species. Therefore, strain KoBa311T is considered to represent a novel species of a new genus, for which the name Desulfoprunum benzoelyticum gen. nov., sp. nov. is proposed. The type strain of Desulfoprunum benzoelyticum is KoBa311T ( = DSM 28570T = KCTC 15441T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.066761-0