RT Journal Article SR Electronic(1) A1 Wang, Hong-Fei A1 Zhang, Yong-Guang A1 Chen, Ji-Yue A1 Hozzein, Wael N. A1 Li, Li A1 Wadaan, Mohammed A. M. A1 Zhang, Yuan-Ming A1 Li, Wen-JunYR 2014 T1 Nesterenkonia rhizosphaerae sp. nov., an alkaliphilic actinobacterium isolated from rhizosphere soil in a saline-alkaline desert JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_12 SP 4021 OP 4026 DO https://doi.org/10.1099/ijs.0.066894-0 PB Microbiology Society, SN 1466-5034, AB An alkaliphilic actinobacterial strain, designated EGI 80099T, was isolated from a rhizosphere soil sample of Reaumuria soongorica found in the desert soils of Fukang, Xinjiang, north-west China. Cells of strain EGI 80099T were Gram-stain-positive, non-motile, non-endospore-forming cocci. The predominant menaquinones were MK-7, MK-8 and MK-9. The major cellular fatty acids (>10 %) were anteiso-C15 : 0 and anteiso-C17 : 0. Analysis of the cell wall showed the presence of peptidoglycan of the type l-Lys–Gly–l-Glu, variation A4α. Cells of the isolate contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unknown phospholipid and an unidentified glycolipid as polar lipids. The genomic DNA G+C content was 63.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain EGI 80099T belongs to the genus Nesterenkonia , sharing 95.68–97.37 % sequence similarities with the type strains of recognized species within this genus. DNA–DNA hybridization of strain EGI 80099T with the type strains of species that showed the highest sequence similarities, Nesterenkonia aethiopica DSM 17733T (97.37 %), Nesterenkonia flava CAAS 251T (97.23 %) and Nesterenkonia xinjiangensis YIM 70097T (97.02 %), gave relatedness values of 8.7–62.2 %. Data from DNA–DNA hybridizations and physiological and biochemical tests indicated that strain EGI 80099T represents a novel species of the genus Nesterenkonia , for which the name Nesterenkonia rhizosphaerae sp. nov. is proposed. The type strain is EGI 80099T ( = BCRC 16947T = JCM 19129T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.066894-0