1887

Abstract

A Gram-stain-positive, non-motile, rod- or coccoid-shaped actinobacterium, designated strain A33, was isolated from a forest soil sample from Nanjing, Jiangsu Province, PR China. The strain grew optimally at 30 °C, pH 7.0 and with 3 % NaCl (w/v). Phylogenetic analysis of the strain, based on 16S rRNA gene sequences, showed that it was most closely related to (98.4 % sequence similarity), (97.5 %), (97.4 %), (97.3 %) and (97.0 %). The major cellular fatty acids were anteiso-C, anteiso-C and iso-C; MK-9(H) was the predominant respiratory quinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and three glycolipids. Cell-wall analysis revealed that the peptidoglycan type was A3α, based on -lysine--alanine; the cell-wall sugars were galactose and mannose. The genomic G+C content of strain A33 was 66.8 mol%. The low DNA–DNA relatedness values between strain A33 and recognized species of the genus and many phenotypic properties supported the classification of strain A33 as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A33 ( = CCTCC AB 2014069 = DSM 28237).

Funding
This study was supported by the:
  • National Nature Science Foundation of China (Award 41201251)
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2015-02-01
2024-03-29
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