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f gen. nov., sp. nov., a moderately thermophilic member of the class in the phylum , and proposal of a new family, fam. nov., and a new order, ord. nov.

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  • Authors: O. L. Kovaleva1 ,  A. Yu. Merkel1 ,  A. A. Novikov2 ,  R. V. Baslerov3 ,  S. V. Toshchakov4 , E. A. Bonch-Osmolovskaya1
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    • 1 1Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya 7/2, 117312 Moscow, Russia 2 2Gubkin Russian State University of Oil and Gas, Leninskiy Prospect 65, 119991 Moscow, Russia 3 3Bioengineering Center, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya 7/1, 117312 Moscow, Russia 4 4Baltic Federal University, Botkina str. 3, 236016 Kaliningrad, Russia
  • O. L. Kovaleva [email protected]
  • First Published Online: 01 February 2015, International Journal of Systematic and Evolutionary Microbiology 65: 549-555, doi: 10.1099/ijs.0.070151-0
  • Subject: New Taxa - Other Bacteria
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gen. nov., sp. nov., a moderately thermophilic member of the class in the phylum , and proposal of a new family, fam. nov., and a new order, ord. nov., Page 1 of 1

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  • Three strains of facultatively aerobic, moderately thermophilic bacteria were isolated from terrestrial hot springs in Baikal Lake region and Kamchatka (Russia). Cells of the new isolates were cocci reproducing by binary fission. The temperature range for growth was between 20 and 56 °C and the pH range for growth from pH 4.5 to 8.5, with optimal growth at 47–50 °C and pH 7.0–7.5. The organisms were chemoheterotrophs preferring sugars and polysaccharides as growth substrates. 16S rRNA gene sequences of strains 2842, 2813 and 2918Kr were nearly identical (99.7–100 % similarity) and indicated that the strains belonged to the phylum . The phylogenetically closest cultivated relatives were 06SJR6-2 and FYK2301M01 with 16S rRNA gene sequence similarity values of 82.4 and 80.3 %, respectively. The novel strains differed from them by higher growth temperature, sensitivity to NaCl concentration above 3.0 % and by their cellular fatty acids profile. On the basis of phylogenetic and physiological data, strains 2842, 2813 and 2918Kr represent a novel genus and species for which we propose the name sp. nov. The type strain is 2842 ( = VKM B-2832 = JCM 19875). We also propose that gen. nov. is the type genus of a novel family, fam. nov. and a novel order, ord. nov.

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 2842, 2813 and 2918Kr are KM036168, KM052380 and KM052379, respectively.

  • A supplementary figure is available with the online Supplementary Material.

  • Abbreviations:
    ECL equivalent chain length

© 2015 IUMS | Published by the Microbiology Society

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