1887

Abstract

A novel anaerobic, extremely thermophilic, facultatively chemolithoautotrophic bacterium designated strain SR was isolated from a terrestrial hot spring in Kamchatka (Russia). The cells of the novel strain were spore-forming rods with a Gram-positive type of cell wall. The novel isolate grew at 60–82 °C (optimum 75 °C) and pH 6.0–7.5 (optimum 6.8). Molecular hydrogen and formate were used as electron donors. Thiosulfate, sulfate or elemental sulfur served as electron acceptors yielding hydrogen sulfide. No growth was observed on either substrate in the presence of nitrate as the electron acceptor. The G+C content of the DNA was 56.2 mol%. Phylogenetic analyses of the 16S rRNA gene showed that strain SR was most closely related to (96.4 % gene sequence similarity). However, the novel isolate possessed phenotypic characteristics that differed significantly from those of , the only other recognized species of the genus . It is concluded that strain SR (=DSM 19636=VKM B-2461) represents the type strain of a novel species of the genus , for which the name sp. nov. is proposed. An emendation of the genus is proposed based on the phenotypic characteristics of the novel species.

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2008-12-01
2024-04-16
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References

  1. Bonch-Osmolovskaya, E. A., Slesarev, A. I., Miroshnichenko, M. L., Svetlichnaya, T. P. & Alekseev, V. A.(1988). Characteristics of Desulfurococcus amylolyticus sp. nov, a new extreme-thermophilic archaebacterium from hot volcanic vents of Kamchatka and Kunashir Island. Microbiology (English translation of Microbiologiia) 57, 78–85. [Google Scholar]
  2. Bonch-Osmolovskaya, E. A., Sokolova, T. G., Kostrikina, N. A. & Zavarzin, G. A.(1990).Desulfurella acetivorans gen. nov. and sp. nov. – a new thermophilic sulfur-reducing eubacterium. Arch Microbiol 153, 151–155.[CrossRef] [Google Scholar]
  3. Bonch-Osmolovskaya, E. A., Miroshnichenko, M. L., Kostrikina, N. A., Chernych, N. A. & Zavarzin, G. A.(1991).Thermoproteus uzoniensis sp. nov., a new extremely thermophilic archaebacterium from Kamchatka continental hot springs. Arch Microbiol 154, 556–559. [Google Scholar]
  4. Bonch-Osmolovskaya, E. A., Miroshnichenko, M. L., Slobodkin, A. I., Sokolova, T. G., Karpov, G. A., Kostrikina, N. A., Zavarzina, D. G., Prokofeva, M. I., Rusanov, I. I. & Pimenov, N. V.(1999). Biodiversity of anaerobic lithotrophic prokaryotes in terrestrial hot springs of Kamchatka. Microbiology (English translation of Microbiologiia) 68, 398–406. [Google Scholar]
  5. Cowen, J. P., Giovannoni, S. J., Kenig, F., Johnson, H. P., Butterfield, D., Rappe, M. S., Hutnak, M. & Lam, P.(2003). Fluids from aging ocean crust that support microbial life. Science 299, 120–123.[CrossRef] [Google Scholar]
  6. Huber, R., Rossnagel, P., Woese, C. R., Rachel, R., Langworthy, T. A. & Stetter, K. O.(1996). Formation of ammonium from nitrate during chemolithoautotrophic growth of the extremely thermophilic bacterium Ammonifex degensii gen. nov., sp. nov. Syst Appl Microbiol 19, 40–49.[CrossRef] [Google Scholar]
  7. Kublanov, I. V., Prokofeva, N. A., Kolganova, T. V., Tourova, T. P., Wiegel, J. & Bonch-Osmolovskaya, E. A.(2007).Thermoanaerobacterium aciditolerans sp. nov., a moderate thermoacidophile from a Kamchatka hot spring. Int J Syst Evol Microbiol 57, 260–264.[CrossRef] [Google Scholar]
  8. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  9. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  10. Miroshnichenko, M. L., Rainey, F. A., Hippe, H., Chernyh, N. A., Kostrikina, N. A. & Bonch-Osmolovskaya, E. A.(1998).Desulfurella kamchatkensis and Desulfurella propionica sp. nov., new sulfur-respiring thermophilic bacteria from Kamchatka thermal environments. Int J Syst Bacteriol 48, 475–479.[CrossRef] [Google Scholar]
  11. Nakagawa, S., Inagaki, F., Suzuki, Y, Steinsbu, B. O., Lever, M. A., Takai, K., Engelen, B., Sako, Y., Wheat, C. G. & Horikoshi, K.(2006). Microbial community in black rust exposed to hot ridge flank crustal fluids. Appl Environ Microbiol 72, 6789–6799.[CrossRef] [Google Scholar]
  12. Perevalova, A. A., Svetlichny, V. A., Kublanov, I. V., Chernyh, N. A., Kostrikina, N. A., Tourova, T. P., Kuznetsov, B. B. & Bonch-Osmolovskaya, E. A.(2005).Desulfurococcus fermentans sp. nov., a novel hyperthermophilic archaeon from a Kamchatka hot spring, and emended description of the genus Desulfurococcus. Int J Syst Evol Microbiol 55, 995–999.[CrossRef] [Google Scholar]
  13. Pfennig, N. & Lippert, K. D.(1966). Über das vitamin B12-bedürfnis phototropher schwefelbacterien. Arch Microbiol 55, 245–256. [Google Scholar]
  14. Trüper, H. G. & Schlegel, H. G.(1964). Sulfur metabolism in Thiorhodaceae. I. Quantitative measurements on growing cells of Chromatium okenii. Antonie van Leeuwenhoek 30, 225–238.[CrossRef] [Google Scholar]
  15. Van de Peer, Y. & De Wachter, R.(1994).treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570. [Google Scholar]
  16. Wolin, E. A., Wolin, M. J. & Wolfe, R. S.(1963). Formation of methane by bacterial extracts. J Biol Chem 238, 2882–2886. [Google Scholar]
  17. Zavarzina, D. G., Zhilina, T. N., Tourova, T. P., Kuznetsov, B. B., Kostrikina, N. A. & Bonch-Osmolovskaya, E. A.(2000).Thermanaerovibrio velox sp. nov., a new anaerobic, thermophilic, organotrophic bacterium that reduces elemental sulfur, and emended description of the genus Thermanaerovibrio. Int J Syst Evol Microbiol 50, 1287–1295.[CrossRef] [Google Scholar]
  18. Zavarzina, D. G., Zhilina, T. N., Tourova, T. P., Kuznetcsov, B. B., Bonch-Osmolovskaya, E. A. & Slobodkin, A. I.(2002).Thermovenabulum ferriorganovorum gen. nov., sp. nov., a novel thermophilic, anaerobic, endospore-forming bacterium. Int J Syst Evol Microbiol 52, 1737–1743.[CrossRef] [Google Scholar]
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