@article{mbs:/content/journal/ijsem/10.1099/ijs.0.2008/000786-0, author = "Huo, Ying-Yi and Wang, Chun-Sheng and Yang, Jun-Yi and Wu, Min and Xu, Xue-Wei", title = "Marinobacter mobilis sp. nov. and Marinobacter zhejiangensis sp. nov., halophilic bacteria isolated from the East China Sea", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2008", volume = "58", number = "12", pages = "2885-2889", doi = "https://doi.org/10.1099/ijs.0.2008/000786-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.2008/000786-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Three Gram-negative, aerobic, motile, halophilic, rod-shaped strains (CN46T, CN71 and CN74T) were isolated from sediment of the East China Sea and subjected to a polyphasic taxonomic study. Strains CN46T and CN71 had identical 16S rRNA gene sequences and phenotypic characteristics. Strain CN46T was moderately halophilic. Growth of strain CN46T was observed between 0.5 and 10.0 % (w/v) NaCl (optimal growth at 3.0–5.0 %) and between pH 6.5 and 9.0. Strain CN74T grew over a wider range of pH (pH 6.0–9.5); the optimum NaCl concentration for growth was 1.0–3.0 %. The major fatty acids of strain CN46T were C16 : 1 ω9c, C16 : 0 and C12 : 0, whereas strain CN74T contained C16 : 0, C16 : 1 ω9c, C18 : 1 ω9c and C12 : 0. The DNA G+C contents of the three isolates were between 58.0 and 58.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains CN46T, CN71 and CN74T grouped together within the cluster of Marinobacter species. 16S rRNA gene sequence similarities of the three strains with the type strains of Marinobacter species ranged from 94.0 to 97.1 %. The DNA–DNA hybridization values of strain CN74T with strains CN46T and CN71 were 35.0 and 36.0 %, respectively. Levels of DNA–DNA relatedness between strains CN46T and CN74T and Marinobacter pelagius CGMCC 1.6775T, Marinobacter gudaonensis CGMCC 1.6294T and Marinobacter koreensis DSM 17924T were 15.3–45.2 %. The results of DNA–DNA hybridizations, fatty acid analysis, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the isolates from closely related species. Two novel species are proposed, named Marinobacter mobilis sp. nov. (type strain CN46T =CGMCC 1.7059T =JCM 15154T) and Marinobacter zhejiangensis sp. nov. (type strain CN74T =CGMCC 1.7061T =JCM 15156T).", }