@article{mbs:/content/journal/ijsem/10.1099/ijs.0.63212-0, author = "Chen, Shuangya and Dong, Xiuzhu", title = "Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill waste water", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2004", volume = "54", number = "6", pages = "2257-2262", doi = "https://doi.org/10.1099/ijs.0.63212-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63212-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Two mesophilic anaerobic bacterial strains (Z7T and Z1) were isolated from waste water sludge of the Xinanzhang paper mill, Beijing, China. The strains were Gram-positive, non-spore-forming and motile. Cells were thin rods (0·2–0·4×4·0–8·0 μm). Growth of the strains was observed at 20–42 °C and pH 5·0–7·5. Both strains hydrolysed gelatin and aesculin and fermented several kinds of mono-, di- and oligosaccharides. The fermentation end products formed from glucose were acetate, ethanol, hydrogen and carbon dioxide. The predominant cellular fatty acids were the branched-chain fatty acids isoC15 : 0 (42·83 %) and isoC14 : 0 (32·11 %). The DNA G+C contents of strains Z7T and Z1 were 50·4 and 48·6 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represent a new phyletic sublineage within the Clostridium leptum rRNA cluster, with <91 % 16S rRNA gene sequence similarity to currently described species. On the basis of polyphasic evidence from this study, Acetanaerobacterium elongatum gen. nov., sp. nov., a novel genus and species, is proposed, with strain Z7T (=JCM 12359T=AS 1.5012T) as the type strain.", }