%0 Journal Article %A Chen, Shuangya %A Dong, Xiuzhu %T Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill waste water %D 2004 %J International Journal of Systematic and Evolutionary Microbiology, %V 54 %N 6 %P 2257-2262 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.63212-0 %I Microbiology Society, %X Two mesophilic anaerobic bacterial strains (Z7T and Z1) were isolated from waste water sludge of the Xinanzhang paper mill, Beijing, China. The strains were Gram-positive, non-spore-forming and motile. Cells were thin rods (0·2–0·4×4·0–8·0 μm). Growth of the strains was observed at 20–42 °C and pH 5·0–7·5. Both strains hydrolysed gelatin and aesculin and fermented several kinds of mono-, di- and oligosaccharides. The fermentation end products formed from glucose were acetate, ethanol, hydrogen and carbon dioxide. The predominant cellular fatty acids were the branched-chain fatty acids isoC15 : 0 (42·83 %) and isoC14 : 0 (32·11 %). The DNA G+C contents of strains Z7T and Z1 were 50·4 and 48·6 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represent a new phyletic sublineage within the Clostridium leptum rRNA cluster, with <91 % 16S rRNA gene sequence similarity to currently described species. On the basis of polyphasic evidence from this study, Acetanaerobacterium elongatum gen. nov., sp. nov., a novel genus and species, is proposed, with strain Z7T (=JCM 12359T=AS 1.5012T) as the type strain. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63212-0