1887

Abstract

A Gram-negative, rod-shaped, psychrophilic, motile, non-spore-forming bacterium, strain U1, was isolated from Ushuaia located at the southernmost tip of Argentina. On the basis of 16S rRNA gene sequence similarity, strain U1 was found to be closely related to (DSM 5604) and (IAM 15010). At the DNA–DNA level, however, the values for similarity were 41 and 25 %, respectively. The major fatty acids present were iso-C, C 7, iso-C and C 7 and the G+C content of the DNA was 43·6 mol%. All of the above characteristics support the affiliation of strain U1 to the genus . Furthermore, on the basis of phenotypic features, chemotaxonomic characteristics and phylogenetic analysis of the 16S rRNA gene sequence, it appears that strain U1 is distinct from the four species with validly published names. Strain U1, therefore, represents a novel species, for which the name sp. nov. is proposed. The type strain of is U1 (=MTCC 6143=DSM 15871=JCM 12170).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63363-0
2005-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/1/ijs550309.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63363-0&mimeType=html&fmt=ahah

References

  1. Baumann L., Baumann P., Mandel M., Allen R. D. 1972; Taxonomy of aerobic marine eubacteria. J Bacteriol 110:402–429
    [Google Scholar]
  2. Baumann P., Gauthier M. J., Baumann L. 1984; Genus Alteromonas, Baumann, Baumann, Mandel and Allen. 1972, 418AL . In Bergey's Manual of Systematic Bacteriology vol 1 pp  343–352 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Felsenstein J. 1995 phylip (phylogeny inference package), version 3.5c Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  4. Gauthier M. J., Breittmayer V. A. 1992; The genera Alteromonas and Marinomonas . In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification Applications vol. 3 pp  3046–3070 Edited by Balows A., Trüper H. G., Dworkin M., Harder H., Schleifer K. H. Berlin: Springer;
    [Google Scholar]
  5. Gauthier G., Gauthier M., Christen R. 1995; Phylogenetic analysis of the genera Alteromonas , Shewanella , and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int J Syst Bacteriol 45:755–761 [CrossRef]
    [Google Scholar]
  6. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  7. MacDonell M. T., Colwell R. R. 1985; Phylogeny of the Vibrionaceae and recommendation for two new genera, Listonella and Shewanella . Syst Appl Microbiol 6:171–182 [CrossRef]
    [Google Scholar]
  8. Reddy G. S. N., Aggarwal R. K., Matsumoto G. I., Shivaji S. 2000; Arthrobacter flavus sp. nov., a psychrophilic bacterium isolate from a pond in McMurdo Dry Valley, Antarctica. Int J Syst Evol Microbiol 50:1553–1561 [CrossRef]
    [Google Scholar]
  9. Reddy G. S. N., Prakash J. S. S., Matsumoto G. I., Stackebrandt E., Shivaji S. 2002; Arthrobacter roseus sp. nov., a psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 52:1017–1021 [CrossRef]
    [Google Scholar]
  10. Romanenko L. A., Uchino M., Mikhailov V. V., Zhukova N. V., Uchimura T. 2003; Marinomonas primoryensis sp. nov., a novel psychrophile isolated from coastal sea-ice in the Sea of Japan. Int J Syst Evol Microbiol 53:829–832 [CrossRef]
    [Google Scholar]
  11. Sato N. S., Murata N. 1988; Membrane lipids. Methods Enzymol 167:251–259
    [Google Scholar]
  12. Shivaji S., Ray M. K., Rao N. S., Saisree L., Jagannadham M. V., Kumar G. S., Reddy G. S. N., Bhargava P. M. 1992; Sphingobacterium antarcticus sp. nov., a psychrotrophic bacterium from the soils of Schirmacher Oasis, Antarctica. Int J Syst Bacteriol 42:102–106 [CrossRef]
    [Google Scholar]
  13. Shivaji S., Bhanu N. V., Aggarwal R. K. 2000; Identification of Yersinia pestis as the causative organism of plague in India as determined by 16S rDNA sequencing and RAPD-based genomic fingerprinting. FEMS Microbiol Lett 189:247–252 [CrossRef]
    [Google Scholar]
  14. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–655 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Solano F., Sanchez-Amat A. 1999; Studies on the phylogenetic relationships of melanogenic marine bacteria: proposal of Marinomonas mediterranea sp. nov. Int J Syst Bacteriol 49:1241–1246 [CrossRef]
    [Google Scholar]
  16. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  17. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  18. Tourova T. P., Antonov A. S. 1987; Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19:333–355
    [Google Scholar]
  19. van Landschoot A., De Ley J. 1983; Intra- and intergeneric similarities of the rRNA cistrons of Alteromonas , Marinomonas (gen. nov.) and some other Gram-negative bacteria. J Gen Microbiol 129:3057–3074
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63363-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63363-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error