1887

Abstract

Thirteen strains of endospore-forming bacteria were isolated from geothermal soils at Cryptogam Ridge, the north-west slope of Mt Melbourne, and at the vents and summit of Mt Rittmann in northern Victoria Land, Antarctica. 16S rRNA gene sequencing, SDS-PAGE and routine phenotypic characterization tests indicated that the seven isolates from the north-west slope of Mt Melbourne represent a novel species of and that the six isolates from Cryptogam Ridge and the vents and summit of Mt Rittmann represent a novel species of . strains were not isolated from the sites at Mt Rittmann or Cryptogam Ridge and strains were not isolated from the north-west slope of Mt Melbourne. Preliminary metabolic studies revealed that -glutamic acid, although not essential for growth, was utilized by both species. The species possessed an uptake system specific for -glutamic acid, whereas the species possessed a more general uptake system capable of transporting other related amino acids. Both species utilized a K antiport system and similar energy systems for the uptake of -glutamic acid. The rate of uptake by the species type strain was 20-fold greater than that shown by the species type strain. The names sp. nov. and sp. nov. are proposed for the novel taxa; the type strains are Logan B-1657 (=LMG 22481=CIP 108307) and Logan B-1599 (LMG 22483=CIP 108308), respectively.

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2005-05-01
2024-03-29
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References

  1. Armienti P., Tripodo A. 1991; Petrography and chemistry of lavas and comagmatic xenoliths of Mt Rittmann, a volcano discovered during the IV Italian expedition in northern Victoria Land (Antarctica). Mem Soc Geol It 46:427–451
    [Google Scholar]
  2. Bargagli R., Broady P. A., Walton D. W. H. 1996; Preliminary investigation of the thermal biosystem of Mount Rittmann fumaroles (northern Victoria Land, Antarctica). Antarct Sci 8:121–126
    [Google Scholar]
  3. Bonaccorso A., Maione M., Pertusati P. C., Privitera E., Ricci C. A. 1991; Fumarolic activity at Mt Rittmann volcano (northern Victoria Land, Antarctica). Mem Soc Geol It 46:453–456
    [Google Scholar]
  4. Broady P. A. 1993; Soils heated by volcanism. In Antarctic Microbiology pp  413–432 Edited by Friedmann E. I. New York: Wiley-Liss;
    [Google Scholar]
  5. Broady P. A., Given D. R., Greenfield L. G., Thompson K. 1987; The biota and environment of fumaroles on Mt Melbourne, northern Victoria Land. Polar Biol 7:97–113 [CrossRef]
    [Google Scholar]
  6. Cruickshank R., Duguid J. P., Marmion B. P., Swain R. H. A. 1975 Medical Microbiology , vol. 2 pp  109 Edinburgh: Churchill Livingstone;
    [Google Scholar]
  7. de Vrij W., Speelmans G., Heyne R. I. R., Konings W. N. 1990; Energy transduction and amino acid transport in thermophilic aerobic and fermentative bacteria. FEMS Microbiol Rev 75:183–200 [CrossRef]
    [Google Scholar]
  8. Dijkhuizen L., Arfman N., Attwood M. M., Brooke A. G., Harder W., Watling E. M. 1988; Isolation and initial characterization of thermotolerant methylotrophic Bacillus strains. FEMS Microbiol Lett 52:209–214 [CrossRef]
    [Google Scholar]
  9. Duguid J. 1951; The demonstration of bacterial capsules and slime. J Pathol Bacteriol 63:673 [CrossRef]
    [Google Scholar]
  10. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  11. Gordon R. E., Haynes W. C., Pang C. H.-N. 1973; Genus Bacillus . Agriculture Handbook no. 427 Washington, DC: United States Department of Agriculture;
    [Google Scholar]
  12. Goto K., Fujita R., Kato Y., Asahara M., Yokota A. 2004; Reclassification of Brevibacillus brevis strains NCIMB 13288 and DSM 6472 (=NRRL NRS-887) as Aneurinibacillus danicus sp.nov. and Brevibacillus limnophilus sp. nov. Int J Syst Evol Microbiol 54:419–427 [CrossRef]
    [Google Scholar]
  13. Heyrman J., Balcaen A., Rodriguez-Diaz M., Logan N. A., Swings J., De Vos P. 2003; Bacillus decolorationis sp. nov., isolated from biodeteriorated parts of the mural paintings at the Servilia tomb (Roman necropolis of Carmona, Spain) and the Saint-Catherine chapel (Castle Herberstein, Austria. Int J Syst Evol Microbiol 53:459–463 [CrossRef]
    [Google Scholar]
  14. Logan N. A., Berkeley R. C. W. 1984; Identification of Bacillus strains using the API system. J Gen Microbiol 130:1871–1882
    [Google Scholar]
  15. Logan N. A., Lebbe L., Hoste B. 7 other authors 2000; Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov. Int J Syst Evol Microbiol 50:1741–1753
    [Google Scholar]
  16. Logan N. A., Forsyth G., Lebbe L. 8 other authors 2002; Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov. Int J Syst Evol Microbiol 52:953–966 [CrossRef]
    [Google Scholar]
  17. Nicolaus B., Marsiglia F., Esposito E., Trincone A., Lama L., Sharp R., di Prisco G., Gambacorta A. 1991; Isolation of five strains of thermophilic eubacteria in Antarctica. Polar Biol 11:425–429 [CrossRef]
    [Google Scholar]
  18. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448 [CrossRef]
    [Google Scholar]
  19. Peddie C. J., Cook G. M., Morgan H. W. 1999; Sodium-dependent glutamate uptake by an alkaliphilic, thermophilic Bacillus strain, TA2.A1. J Bacteriol 181:3172–3177
    [Google Scholar]
  20. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Modern Microbial Methods ( Chemical Methods in Prokaryotic Systematics Series ) pp  493–521 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  21. Shida O., Takagi H., Kadowaki K., Komagata K. 1996; Proposal for two new genera. Brevibacillus gen. nov. and Aneurinibacillus gen. nov.. Int J Syst Bacteriol 46:939–946 [CrossRef]
    [Google Scholar]
  22. Siebert J., Hirsch P. 1988; Characterization of 15 selected coccal bacteria isolated from Antarctic rock and soil samples from the McMurdo-Dry Valleys (South-Victoria Land). Polar Biol 9:37–44 [CrossRef]
    [Google Scholar]
  23. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  24. Stackebrandt E., Frederiksen W., Garrity G. M. 10 other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  25. Suzuki K., Goodfellow M., O'Donnell A. G. 1993; Cell envelopes and classification. In Handbook of New Bacterial Systematics pp  195–250 Edited by Goodfellow M., O'Donnell A. G. London: Academic Press;
    [Google Scholar]
  26. Vauterin L., Vauterin P. 1992; Computer aided objective comparison of electrophoretic patterns for grouping and identification of microorganisms. Eur Microbiol 1:37–41
    [Google Scholar]
  27. Willems A., Doignon-Bourcier F., Goris J., Coopman R., de Lajudie P., De Vos P., Gillis M. 2001; DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51:1315–1322
    [Google Scholar]
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