RT Journal Article SR Electronic(1) A1 Kim, Hong A1 Kim, Sun-Hyun A1 Shim, Tae-Sun A1 Kim, Mi-na A1 Bai, Gill-Han A1 Park, Young-Gil A1 Lee, Sueng-Hyun A1 Chae, Gue-Tae A1 Cha, Chang-Yong A1 Kook, Yoon-Hoh A1 Kim, Bum-JoonYR 2005 T1 Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65) JF International Journal of Systematic and Evolutionary Microbiology, VO 55 IS 4 SP 1649 OP 1656 DO https://doi.org/10.1099/ijs.0.63553-0 PB Microbiology Society, SN 1466-5034, AB The nucleotide sequences (604 bp) of partial heat-shock protein genes (hsp65) from 161 Mycobacterium strains containing 56 reference Mycobacterium species and 105 clinical isolates were determined and compared. hsp65 sequence analysis showed a higher degree of divergence between Mycobacterium species than did 16S rRNA gene analysis. Generally, the topology of the phylogenetic tree based on the hsp65 DNA sequences was similar to that of the 16S rRNA gene, thus revealing natural relationships among Mycobacterium species. When a direct sequencing protocol targeting 422 bp sequences was applied to 70 non-tuberculous mycobacterium (NTM) clinical isolates, all NTMs were clearly identified. In addition, an XhoI PCR restriction fragment length polymorphism analysis method for the differentiation of Mycobacterium tuberculosis complex from NTM strains was developed during this study. The results obtained suggest that 604 bp hsp65 sequences are useful for the phylogenetic analysis and species identification of mycobacteria., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63553-0