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Volume 55, Issue 4

sp. nov., a novel Na-requiring and hexahydro-1,3,5-trinitro-1,3,5-triazine-degrading bacterium from marine sediment Free

Abstract

Previously, a psychrophilic rod-shaped marine bacterium (strain HAW-EB3) isolated from Halifax Harbour sediment was noted for its ability to degrade hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). In the present study phenotypic, chemotaxonomic and genotypic characterization showed that strain HAW-EB3 represents a novel species of . Strain HAW-EB3 contained lysine decarboxylase, which is absent in other known species, and distinguished itself from most other species of by the presence of arginine dehydrolase, ornithine decarboxylase and chitinase, and by its ability to oxidize and ferment -acetyl--glucosamine. Strain HAW-EB3 grew on several carbon sources (-acetyl--glucosamine, Tween 40, Tween 80, acetate, succinate, butyrate and serine) and showed distinctive fatty acid and quinone compositions. Both phenotypic and 16S rRNA gene phylogenetic cluster analyses demonstrated that HAW-EB3 belongs to the Na-requiring group of species. The HAW-EB3 16S rRNA gene sequence displayed ⩽97·4 % similarity to all known species and was most similar to those of two bioluminescent species, and . However, of strain HAW-EB3 was significantly different from those of other species, with similarities less than 85 %. DNA-DNA hybridization showed that its genomic DNA was less than 25 % related to that of or . Therefore we propose sp. nov., with HAW-EB3 (=NCIMB 14036=DSM 17055) as the type strain.

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Keyword(s): MK, menaquinone , MMK, methylmenaquinone and RDX, hexahydro-1,3,5-trinitro-1,3,5-triazine
SGM
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2005-07-01
2024-04-24
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Shewanella sediminis sp. nov., a novel Na+-requiring and hexahydro-1,3,5-trinitro-1,3,5-triazine-degrading bacterium from marine sediment
Int J Syst Evol Microbiol 55, 1511 (2005); https://doi.org/10.1099/ijs.0.63604-0
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