RT Journal Article SR Electronic(1) A1 Amakata, Daiki A1 Matsuo, Yasuhiro A1 Shimono, Kumiko A1 Park, Jae Kweon A1 Yun, Choong Soo A1 Matsuda, Hideyuki A1 Yokota, Akira A1 Kawamukai, MakotoYR 2005 T1 Mitsuaria chitosanitabida gen. nov., sp. nov., an aerobic, chitosanase-producing member of the ‘Betaproteobacteria’ JF International Journal of Systematic and Evolutionary Microbiology, VO 55 IS 5 SP 1927 OP 1932 DO https://doi.org/10.1099/ijs.0.63629-0 PB Microbiology Society, SN 1466-5034, AB Four strains (3001T, 2, 12 and 13), which were isolated as chitosanase-producing bacteria from soil from Matsue city (Japan), were studied phenotypically, genotypically and phylogenetically. Based on sequence analysis of 16S rRNA genes, DNA G+C content (67·4–69·2 mol%), quinone type (UQ-8), major fatty acid composition (3-OH 10 : 0, 3-OH 14 : 0) and other phylogenetic studies, strains 3001T, 12 and 13 were found to occupy a separate position in the ‘Betaproteobacteria’. Roseateles depolymerans, Rubrivivax gelatinosus and Ideonella dechloratans were their closest neighbours (93–95 % 16S rRNA gene sequence similarity). The 16S rRNA gene sequence and other characteristics suggested that strain 2 belonged to the genus Flavobacterium. DNA–DNA hybridization experiments supported the conclusion that strains 3001T, 12 and 13 were of the same species (72–78 % DNA hybridization) and only distantly related to I. dechloratans and R. gelatinosus. It is proposed that strains 3001T, 12 and 13 represent a novel genus and species for which the name Mitsuaria chitosanitabida gen. nov., sp. nov. is proposed. The type strain of Mitsuaria chitosanitabida is 3001T (=IAM 14711T=ATCC BAA-476T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63629-0