Nocardioides dubius sp. nov., isolated from an alkaline soil
Yoon, Jung-Hoon and Lee, Choong-Hwan and Oh, Tae-Kwang,, 55, 2209-2212 (2005),
doi = https://doi.org/10.1099/ijs.0.63748-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A Gram-positive, rod- or coccus-shaped bacterial strain, KSL-104T, was isolated from an alkaline soil from Korea and its taxonomic position was investigated by a polyphasic approach. Strain KSL-104T grew optimally at pH 7·0–8·0 and 30 °C. It was characterized chemotaxonomically as having a cell-wall peptidoglycan type based on ll-2,6-diaminopimelic acid with MK-8(H4) as the predominant menaquinone. The major fatty acid was iso-C16 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The DNA G+C content was 70·6 mol%. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain KSL-104T joined the cluster comprising Nocardioides jensenii and Marmoricola aurantiacus. The cellular fatty acid profile of strain KSL-104T was different from that of M. aurantiacus. Strain KSL-104T and the type strain of N. jensenii exhibited a 16S rRNA gene sequence similarity value of 97·1 % and a mean DNA–DNA relatedness value of 13 %. Levels of 16S rRNA gene sequence similarity between strain KSL-104T and the type strains of other Nocardioides species were in the range 94·0–96·5 %. On the basis of phenotypic, phylogenetic and genetic data, strain KSL-104T (=KCTC 9992T=JCM 13008T) represents a novel species of the genus Nocardioides, for which the name Nocardioides dubius sp. nov. is proposed.,
language=,
type=