RT Journal Article SR Electronic(1) A1 Takahashi, Yōko A1 Matsumoto, Atsuko A1 Morisaki, Kurimi A1 Ōmura, SatoshiYR 2006 T1 Patulibacter minatonensis gen. nov., sp. nov., a novel actinobacterium isolated using an agar medium supplemented with superoxide dismutase, and proposal of Patulibacteraceae fam. nov. JF International Journal of Systematic and Evolutionary Microbiology, VO 56 IS 2 SP 401 OP 406 DO https://doi.org/10.1099/ijs.0.63796-0 PB Microbiology Society, SN 1466-5034, AB A novel Gram-positive bacterial strain, designated KV-614T, was isolated from a soil sample using an agar medium supplemented with superoxide dismutase. Based on 16S rRNA gene sequence analysis, it was found that the strain represented a novel deep-rooting lineage within the class Actinobacteria and clustered with yet-uncultivated bacteria from terrestrial environments and some unidentified strains isolated by unique methods. The most closely related established genus was Conexibacter (92·4 % sequence similarity to Conexibacter woesei DSM 14684T). Cells of strain KV-614T were rod-shaped and motile with long flagella. The strain was catalase-positive, oxidase-negative and grew under aerobic conditions. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid and alanine and glutamic acid. The peptidoglycan acyl type was acetyl. The only detected isoprenoid quinone was demethylmenaquinone with seven isoprene units (DMK-7). Mycolic acids were not detected. The predominant cellular fatty acid was ω9c-octadecenoic acid (C18 : 1ω9c). Minor components were 12-methyl tetradecanoic acid (anteiso-C15 : 0) and 14-methyl hexadecanoic acid (anteiso-C17 : 0). The DNA G+C content was 72 mol%. On the basis of phenotypic and genotypic characteristics, it is proposed that strain KV-614T represents a new genus and a novel species, Patulibacter minatonensis gen. nov., sp. nov., in the class Actinobacteria. The type strain is KV-614T (=NRRL B-24346T=JCM 12834T=NBRC 100761T). The creation of the family Patulibacteraceae fam. nov. is proposed to encompass the genus Patulibacter gen. nov., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63796-0