@article{mbs:/content/journal/ijsem/10.1099/ijs.0.63887-0, author = "Ma, Kai and Liu, Xiaoli and Dong, Xiuzhu", title = "Methanosaeta harundinacea sp. nov., a novel acetate-scavenging methanogen isolated from a UASB reactor", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2006", volume = "56", number = "1", pages = "127-131", doi = "https://doi.org/10.1099/ijs.0.63887-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63887-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "UASB, upflow anaerobic sludge blanket", abstract = "Two methanogenic strains, 8AcT and 6Ac, were isolated from an upflow anaerobic sludge blanket reactor treating beer-manufacture wastewater in Beijing, China. Cells of strains 8AcT and 6Ac were rod-shaped (0·8–1·0×3–5 μm) and non-motile, occurring singly or in pairs; however, at high cell density the cells were arranged in long chains within a common sheath. The two strains used acetate exclusively for growth and methane production. The specific growth rate of strain 8AcT was 0·030 h−1 when growing in acetate (20 mM) at 37 °C. The temperature range for growth was 25–45 °C, with the fastest growth at 34–37 °C. The pH range for growth and methane production was 6·5–9·0, with the fastest growth at pH 7·2–7·6. The G+C content of genomic DNA of strain 8AcT was 55·7 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that the novel strains clustered with Methanosaeta species; the 16S rRNA gene sequence similarities between strain 8AcT and Methanosaeta concilii DSM 3013 and ‘Methanosaeta thermophila’ DSM 6194 were 92·5 and 87·3 %, respectively. The sequence similarity levels of mcrA, the gene encoding the α-subunit of methyl-coenzyme M reductase, and of the deduced amino acids of mcrA, between strain 8AcT and Methanosaeta concilii DSM 3671T were 36 and 78·9 %, respectively. Based on the phylogenetic and phenotypic analyses, the novel species Methanosaeta harundinacea sp. nov. is proposed, with strain 8AcT (=JCM 13211T=CGMCC 1.5026T) as the type strain.", }