RT Journal Article SR Electronic(1) A1 Ma, Kai A1 Liu, Xiaoli A1 Dong, XiuzhuYR 2006 T1 Methanosaeta harundinacea sp. nov., a novel acetate-scavenging methanogen isolated from a UASB reactor JF International Journal of Systematic and Evolutionary Microbiology, VO 56 IS 1 SP 127 OP 131 DO https://doi.org/10.1099/ijs.0.63887-0 PB Microbiology Society, SN 1466-5034, AB Two methanogenic strains, 8AcT and 6Ac, were isolated from an upflow anaerobic sludge blanket reactor treating beer-manufacture wastewater in Beijing, China. Cells of strains 8AcT and 6Ac were rod-shaped (0·8–1·0×3–5 μm) and non-motile, occurring singly or in pairs; however, at high cell density the cells were arranged in long chains within a common sheath. The two strains used acetate exclusively for growth and methane production. The specific growth rate of strain 8AcT was 0·030 h−1 when growing in acetate (20 mM) at 37 °C. The temperature range for growth was 25–45 °C, with the fastest growth at 34–37 °C. The pH range for growth and methane production was 6·5–9·0, with the fastest growth at pH 7·2–7·6. The G+C content of genomic DNA of strain 8AcT was 55·7 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that the novel strains clustered with Methanosaeta species; the 16S rRNA gene sequence similarities between strain 8AcT and Methanosaeta concilii DSM 3013 and ‘Methanosaeta thermophila’ DSM 6194 were 92·5 and 87·3 %, respectively. The sequence similarity levels of mcrA, the gene encoding the α-subunit of methyl-coenzyme M reductase, and of the deduced amino acids of mcrA, between strain 8AcT and Methanosaeta concilii DSM 3671T were 36 and 78·9 %, respectively. Based on the phylogenetic and phenotypic analyses, the novel species Methanosaeta harundinacea sp. nov. is proposed, with strain 8AcT (=JCM 13211T=CGMCC 1.5026T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63887-0