1887

Abstract

A Gram-positive, spore-forming bacillus was isolated from a sample taken from an approximately 2000-year-old shaft-tomb located in the Mexican state of Jalisco, near the city of Tequila. Tentative identification using conventional biochemical analysis consistently identified the isolate as . DNA isolated from the tomb isolate, strain 10b, and closely related species was used to amplify a -specific portion of the highly conserved 16S rRNA gene and an internal region of the superoxide dismutase gene ( ). Trees derived from maximum-likelihood methods applied to the sequences yielded non-zero branch lengths between strain 10b and its closest relative, whereas a comparison of a -specific 546 bp amplicon of the 16S rRNA gene demonstrated 99 % similarity with . Although the 16S rRNA gene sequences of strain 10b and were 99 % similar, PFGE of I-digested DNA of strain 10b revealed a restriction profile that was considerably different from those of and other closely related species. Whereas qualitative differences in whole-cell fatty acids were not observed, significant quantitative differences were found to exist between strain 10b and each of the other closely related species examined. In addition, DNA–DNA hybridization studies demonstrated that strain 10b had a relatedness value of less than 70 % with and other closely related species. Evidence from the sequences, whole-cell fatty acid profiles and PFGE analysis, together with results from DNA–DNA hybridization studies, justify the classification of strain 10b as representing a distinct species, for which the name sp. nov. is proposed. The type strain is 10b (=ATCC BAA-819=NCTC 13306).

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2006-07-01
2024-03-28
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