1887

Abstract

Two bacterial strains, DSM 20253 and DSM 20254, isolated from rennin and regarded as causing cheese spoilage, were deposited in the DSMZ as sp. by J. Stadhouders. The strains show 99·9 % 16S rRNA gene sequence similarity and have less than 94·3 % similarity with any other species of the genus. is their closest phylogenetic neighbour. DNA–DNA hybridization experiments confirmed that the two strains are members of the same species with separate status within the genus . The strains are homofermentative lactic acid bacteria and can be phenotypically and genotypically distinguished from their closest relatives. 16S rRNA gene-targeted specific primers were designed to enable PCR detection of the novel species. The name sp. nov. is proposed for the novel isolates, with strain CECT 5922 (=DSM 20253) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64021-0
2006-02-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/2/449.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64021-0&mimeType=html&fmt=ahah

References

  1. Abo-Elnaga I. G., Kandler O. 1965; Zur Taxonomie der Gattung Lactobacillus Beijerinck. I. Das Subgenus Streptobacterium Orla-Jensen. Zentralbl Bakteriol Parasitkd Infektionskr Hyg 119:1–36 (in German
    [Google Scholar]
  2. Alm E. W., Oerther D. B., Larsen N., Stahl D. A., Raskin L. 1996; The oligonucleotide probe database. Appl Environ Microbiol 62:3557–3559
    [Google Scholar]
  3. Cachat E., Priest F. G. 2005; Lactobacillus suntoryeus sp. nov., isolated from malt whisky distilleries. Int J Syst Evol Microbiol 55:31–34 [CrossRef]
    [Google Scholar]
  4. Chenoll E., Macián M. C., Aznar R. 2003; Identification of Carnobacterium , Lactobacillus , Leuconostoc and Pediococcus by rDNA-based techniques. Syst Appl Microbiol 26:546–556 [CrossRef]
    [Google Scholar]
  5. Dicks L. M. T., Silvester M., Lawson P. A., Collins M. D. 2000; Lactobacillus fornicalis sp. nov., isolated from the posterior fornix of the human vagina. Int J Syst Evol Microbiol 50:1253–1258 [CrossRef]
    [Google Scholar]
  6. Edwards C. G., Haag K. M., Collins M. D., Hutson R. A., Huang Y. C. 1998; Lactobacillus kunkeei sp. nov.: a spoilage organism associated with grape juice fermentations. J Appl Microbiol 84:698–702 [CrossRef]
    [Google Scholar]
  7. Garrity G. M., Bell J. A., Lilburn T. G. 2004; Taxonomic outline of the Prokaryotes. In Bergey's Manual of Systematic Bacteriology , 2nd edn, release 5.0. New York: Springer; DOI: 10.1007/bergeysoutline200405
    [Google Scholar]
  8. Hammes W. P., Vogel R. F. 1995; The genus Lactobacillus . In The Lactic Acid Bacteria , vol. 2, The Genera of Lactic Acid Bacteria . pp  19–54 Edited by Wood B. J. B., Holzapfel W. H. London: Blackie Academic & Professional;
  9. Kato Y., Sakala R. M., Hayashidani H., Kiuchi A., Kaneuchi C., Ogawa M. 2000; Lactobacillus algidus sp. nov., a psychrophilic lactic acid bacterium isolated from vacuum-packaged refrigerated beef. Int J Syst Evol Microbiol 50:1143–1149 [CrossRef]
    [Google Scholar]
  10. Ludwig W., Strunk O., Klugbauer S., Klugbauer N., Weizenegger M., Neumaier J., Bachleitner M., Schleifer K. H. 1998; Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19:554–568 [CrossRef]
    [Google Scholar]
  11. Ludwig W., Strunk O., Westram R. 29 other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  12. Macián M. C., Chenoll E., Aznar R. 2004; Simultaneous detection of Carnobacterium and Leuconostoc in meat products by multiplex PCR. J Appl Microbiol 97:384–394 [CrossRef]
    [Google Scholar]
  13. Mukai T., Arihara K., Ikeda A., Nomura K., Suzuki F., Ohori H. 2003; Lactobacillus kitasatonis sp. nov., from chicken intestine. Int J Syst Evol Microbiol 53:2055–2059 [CrossRef]
    [Google Scholar]
  14. Sakala R. M., Kato Y., Hayashidani H., Murakami M., Kaneuchi C., Ogawa M. 2002; Lactobacillus fuchuensis sp. nov., isolated from vacuum-packaged refrigerated beef. Int J Syst Evol Microbiol 52:1151–1154 [CrossRef]
    [Google Scholar]
  15. Stadhouders J., Veringa H. A. 1967; Texture and flavour defects in cheese caused by bacteria from contaminated rennet. Neth Milk & Dairy J 21:192–207
    [Google Scholar]
  16. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. 1998; Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64021-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64021-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error