1887

Abstract

A Gram-positive, aerobic, rod-shaped, motile, endospore-forming bacterium was isolated from pasteurized milk from Bavaria, Germany. 16S rRNA gene sequence similarities indicated that strain WSBC 24001 was most closely related to species (95.3–96.1 %), species (95.6–95.7 %), IAM 12464 (95.5 %) and IFO 15566 (95.2 %). However, strain WSBC 24001 showed the highest level of sequence similarity to an unnamed strain, MB-9 (97.6 %), which was isolated from coastal surface sediments in California. Hence, this strain was included in our study. The genomic DNA G+C contents of strains WSBC 24001 and MB-9 were 36.4 mol and 40.8 mol%, respectively. The major respiratory quinone of both strains was menaquinone MK-7 and the peptidoglycan type was A4 (-orn←-Asp). The polar lipid profiles of these strains contained a predominance of diphosphatidylglycerol and moderate to minor amounts of phosphatidylglycerol, an unknown phospholipid and an unknown aminophospholipid. However, strain WSBC 24001 could be distinguished from strain MB-9 by the presence of an unknown lipid. The fatty acid profiles of the two strains comprised mainly iso- and anteiso-branched acids, but showed some significant quantitative differences in the amounts of certain acids. The DNA–DNA relatedness value (15.5 %) clearly demonstrated that strains WSBC 24001 and MB-9 are representatives of two different species. On the basis of their phylogenetic position and morphological, physiological and chemotaxonomic properties, a novel genus is proposed, gen. nov., with two novel species, the type species sp. nov. (type strain WSBC 24001=DSM 15681=CCM 7096) and sp. nov. (type strain MB-9=DSM 16628=CCM 7237).

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2006-06-01
2024-03-28
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