1887

Abstract

Six strains of lactic acid bacteria (LAB) were isolated from a ripe fig. These strains constituted a highly homogeneous, but distinct, cluster that was separate from other LAB species in a polyphasic approach including dot-blot DNA–DNA hybridization, SDS-PAGE whole-cell protein profiling, carbohydrate fermentation ability, growth characteristics, enzymic profiling, pulsed-field gel electrophoresis macrorestriction analysis and RFLPs. Phylogenetic analysis based on 16S rRNA gene sequencing positioned a representative strain, LC51, in a distinct line of descent within the recently described clade comprising , and ; was its closest neighbour (98 % sequence similarity). DNA–DNA hybridization values and chemotaxonomic and biochemical characteristics, including enzymic profiles detected with API ZYM microtubes, confirmed that this group of strains is distinct from and represents a novel species within the genus . Taking into account the common origin and phylogenetic proximity, the name sp. nov. is proposed. Strain LC51 (=DSM 15468=CECT 5759) is the type strain; the DNA G+C content of this strain is 44.5 mol%.

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2006-06-01
2024-04-19
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