1887

Abstract

Sixteen Gram-negative, rod-shaped, non-spore-forming isolates were obtained from a nitrifying inoculum. Analysis of repetitive sequence-based PCR and SDS-PAGE banding patterns, 16S rRNA gene sequence analysis and DNA–DNA hybridizations showed that the isolates belonged to various groups within the genus . One group of isolates could be assigned to and a second to . Two groups could be differentiated genotypically from each other and from all other currently known species. Analysis of the fatty acid composition and physiological and biochemical tests allowed differentiation of these groups from their closest phylogenetic neighbours and they therefore represent two novel species within the genus , for which the names sp. nov. and sp. nov. are proposed, with strains LMG 23201 (=DSM 17833=R-20805) and LMG 23199 (=DSM 17834=R-20821), respectively, as the type strains.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64224-0
2006-08-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/8/1875.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64224-0&mimeType=html&fmt=ahah

References

  1. Anzai Y., Kudo Y., Oyaizu H. 1997; The phylogeny of the genera Chryseomonas , Flavimonas , and Pseudomonas supports synonymy of these three genera. Int J Syst Bacteriol 47:249–251 [CrossRef]
    [Google Scholar]
  2. Anzai Y., Kim H., Park J.-Y., Wakabayashi H., Oyaizu H. 2000; Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50:1563–1589 [CrossRef]
    [Google Scholar]
  3. Elomari M., Cordoler L., Hoste B., Gillis M., Izard D., Leclerc H. 1996; DNA relatedness among Pseudomonas strains isolated from natural mineral waters and proposal of Pseudomonas veronii sp. nov. Int J Syst Bacteriol 46:1138–1144 [CrossRef]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Grommen R., Van Hauteghem I., Van Wambeke M., Verstraete W. 2002; An improved nitrifying enrichment to remove ammonium and nitrite from freshwater aquaria systems. Aquaculture 211:115–124 [CrossRef]
    [Google Scholar]
  6. Heyrman J., Verbeeren J., Schumann P., Swings J., De Vos P. 2005; Six novel Arthrobacter species isolated from deteriorated mural paintings. Int J Syst Evol Microbiol 55:1457–1464 [CrossRef]
    [Google Scholar]
  7. Hildebrand D. C., Palleroni N. J., Hendson M., Toth J., Johnson J. L. 1994; Pseudomonas flavescens sp. nov., isolated from walnut blight cankers. Int J Syst Bacteriol 44:410–415 [CrossRef]
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  10. King E. O., Ward M. K., Rainey D. E. 1954; Two simple media for the demonstration of pyocyanin and fluorescein. J Lab Clin Med 44:301–307
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  12. Pearson W. R. 1990; Rapid and sensitive sequence comparison with fastp and fasta. Methods Enzymol 183:63–98
    [Google Scholar]
  13. Peix A., Berge O., Rivas R., Abril A., Velázquez E. 2005; Pseudomonas argentinensis sp. nov., a novel yellow pigment-producing bacterial species, isolated from rhizospheric soil in Córdoba, Argentina. Int J Syst Evol Microbiol 55:1107–1112 [CrossRef]
    [Google Scholar]
  14. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics pp  493–521 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  15. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43:159–271 [CrossRef]
    [Google Scholar]
  16. Uchino M., Kosako Y., Uchimura T., Komagata K. 2000; Emendation of Pseudomonas straminea Iizuka and Komagata 1963. Int J Syst Evol Microbiol 50:1513–1519 [CrossRef]
    [Google Scholar]
  17. Vandamme P., Vancanneyt M., Pot B. 10 other authors 1992; Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42:344–356 [CrossRef]
    [Google Scholar]
  18. Vanparys B., Heylen K., Lebbe L., De Vos P. 2005; Pedobacter caeni sp. nov., a novel species isolated from a nitrifying inoculum. Int J Syst Evol Microbiol 55:1315–1318 [CrossRef]
    [Google Scholar]
  19. Verhille S., Baida N., Dabboussi F., Hamze M., Izard D., Leclerc H. 1999; Pseudomonas gessardii sp. nov. and Pseudomonas migulae sp. nov. two new species isolated from natural mineral waters. Int J Syst Bacteriol 49:1559–1572 [CrossRef]
    [Google Scholar]
  20. Wakabayashi H., Egusa S. 1972; Characteristics of a Pseudomonas sp. from an epizootic of pond-cultured eels ( Anguilla japonica ). Bull Jpn Soc Sci Fish 38:577–587 [CrossRef]
    [Google Scholar]
  21. Willems A., Doignon-Bourcier F., Goris J., Coopman R., de Lajudie P., De Vos P., Gillis M. 2001; DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51:1315–1322
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64224-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64224-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error