1887

Abstract

Two novel exopolysaccharide-producing bacteria, strains S18K6 and S18K5, were isolated from Pacific Ocean sediment. The isolates were Gram-negative, motile, strictly aerobic chemoheterotrophic bacteria. The DNA G+C contents of strains S18K6 and S18K5 were 44.8 and 46.3 mol%, respectively. DNA–DNA relatedness between the two strains was 70 %. Major fatty acids were hexadecanoic acid (C), hexadecenoic acid (C 7) and octadecenoic acid (C 7). 16S rRNA gene sequence, chemotaxonomic and morphological data indicated that these strains clearly belonged to the genus . Based on phenotypic properties and DNA–DNA hybridization data, strains S18K6 and S18K5 are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S18K6 (=JCM 13645=NCIMB 14146).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64413-0
2006-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/12/2883.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64413-0&mimeType=html&fmt=ahah

References

  1. Bowman J. P., McCammon S. A., Brown J. L., McMeekin T. A. 1998; Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov., psychrophilic bacteria from Antarctic sea-ice habitats. Int J Syst Bacteriol 48:1213–1222 [CrossRef]
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  3. Ivanova E. P., Zhukova N. V., Svetashev V. I., Gorshkova N. M., Kurilenko V. V., Frolova G. M., Mikhailov V. V. 2000; Evaluation of phospholipids and fatty acid composition as chemotaxonomic markers of Alteromonas -like proteobacteria. Curr Microbiol 41:341–345 [CrossRef]
    [Google Scholar]
  4. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  5. Komagata K. 1985; Bacteria(1) – the aerobic bacteria. In Classification and Identification of Microorganisms vol 2 pp  99–161 Edited by Hasegawa T. Tokyo: Gakkai Shuppan;
    [Google Scholar]
  6. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  7. Romanenko L. A., Zhukova N. V., Rohde M., Lysenko A. M., Mikhailov V. V., Stackebrandt E. 2003; Glaciecola mesophila sp. nov., a novel marine agar-digesting bacterium. Int J Syst Evol Microbiol 53:647–651 [CrossRef]
    [Google Scholar]
  8. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  9. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  10. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  11. Van Trappen S., Tan T.-L., Yang J., Mergaert J., Swings J. 2004; Glaciecola polaris sp. nov., a novel budding and prosthecate bacterium from the Arctic Ocean, and emended description of the genus Glaciecola . Int J Syst Evol Microbiol 54:1765–1771 [CrossRef]
    [Google Scholar]
  12. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64413-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64413-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error