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Volume 57, Issue 1

gen. nov., sp. nov., a novel marine alphaproteobacterium isolated from coastal sediment in Qingdao, China Free

Abstract

The taxonomic position of strain LMG 23452, which was isolated from coastal sediment from an aquaculture site near Qingdao, China, in 2000, was determined. Strain LMG 23452 comprised Gram-negative, non-spore-forming, motile rods and was found to be a halotolerant, aerobic, chemoheterotroph that produces catalase and oxidase. Comparative 16S rRNA gene sequence analysis revealed that strain LMG 23452 shared approximately 89 % sequence similarity with members of the genera , , and , which belong to two different orders within the . Further phylogenetic analysis of the 16S rRNA gene sequence showed that strain LMG 23452 formed a separate branch within the order , falling between the genera and of the families and , respectively. Strain LMG 23452 could be differentiated from its closest phylogenetic neighbours on the basis of several phenotypic features, including hydrolysis of the substrates starch and casein and assimilation of the carbohydrates -glucose, -mannose, mannitol, maltose and -arabinose, and chemotaxonomically by the presence of the fatty acids C 3-OH, C 11, C 5 and C 5. The major fatty acids detected in strain LMG 23452 were C 7, C, C cyclo 8, C 7 and C 6 and the G+C content of the genomic DNA was 57.1 mol%. Therefore, the polyphasic data support the placement of strain LMG 23452 within a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is LMG 23452 (=CBMAI 737).

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2007-01-01
2024-04-20
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Sneathiella chinensis gen. nov., sp. nov., a novel marine alphaproteobacterium isolated from coastal sediment in Qingdao, China
Int J Syst Evol Microbiol 57, 114 (2007); https://doi.org/10.1099/ijs.0.64478-0
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